延边地区牛瑟氏泰勒虫病的分子流行病学调查  

Molecular epidemiological survey of Theileria sergenti infections in cattle in Yanbian Prefecture

作　　者：胡诗悦[1] 钱年超[1] 王轶男[1] 贾立军[1] 张守发[1] 

机构地区：[1]延边大学农学院动物医学系,吉林延吉133002

出　　处：《中国兽医科学》2012年第6期647-650,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30960278)

摘　　要：为了解吉林省延边地区牛瑟氏泰勒虫病的流行情况,分别采用血涂片染色镜检法和PCR方法对采自延边地区的206份牛血液样本进行了牛瑟氏泰勒虫病的分子流行病学调查。结果显示,PCR检测的阳性率为62.6%,显著高于血涂片染色镜检法检测的阳性率(17.5%)。采用统计学分析方法对PCR检测的206份血液样本进行了不同地区、不同年龄、不同品种及饲养方式的比较;结果,不同地区之间和不同品种之间瑟氏泰勒虫感染率差异不显著(P>0.05);而不同年龄阶段之间和不同饲养方式之间牛瑟氏泰勒虫感染率差异显著(P<0.05)。结果表明,吉林省延边地区是牛瑟氏泰勒虫病的流行地区。In order to understand the prevalence of Theileria sergenti infections in cattle in Yanbian area,a molecular epidemiological survey was conducted to examine T. sergenti using PCR method. Totally, 206 blood samples were detected. In result,the positive rate of PCR method was 62.6% ,which was significantly higher than 17.5% by microscopic examination of Giemsa-stained blood smears. Risk factors including counties,ages,breeds and grazing were evaluated and statistical analyzed. In result, ages and grazing were associated with the prevalence of T. sergenti infections（P〈0.05） ,while counties and breeds were not （P〉0.05）. The survey confirmed that Yanbian Prefecture was the epidemic region of T. sergenti.

关 键 词：瑟氏泰勒虫 PCR 血涂片镜检法 分子流行病学调查 

分 类 号：S852.729[农业科学—基础兽医学]