褪黑素对绒山羊皮肤FGF5等绒毛生长相关基因的影响  

Effect of Melatonin on Genes Including FGF5 That Related to Hair Growth in Cashmere Goat Skin

作　　者：赵德超[1] 付绍印[1] 吕晓曼[1] 吴江鸿[1] 张燕军[1] 李金泉[1] 王志新[1] 张永斌[1] 张文广[1] 

机构地区：[1]内蒙古农业大学动物科学学院动物遗传育种与繁殖自治区重点实验室,呼和浩特010018

出　　处：《中国生物工程杂志》2012年第6期20-26,共7页China Biotechnology

基　　金：国家科技支撑项目(2011BAD28B05);国家自然科学基金(30960246)

摘　　要：目的:旨在分析埋植褪黑素后对绒山羊皮肤绒毛生长相关基因表达的影响,进而探讨褪黑素促绒毛生长的机理。方法:以内蒙古白绒山羊为研究对象,选取体况相近的4月龄母羔羊10只,按试验要求随机分为2组,处理组按照2mg/kg BW(体重)的剂量每两个月耳后皮下埋植褪黑素,对照组不埋植,试验时间为1年。实验开始后每月采集绒山羊体侧皮肤和毛样,利用荧光定量PCR技术检测9个绒毛生长相关基因的表达变化。结果:(1)埋植褪黑素后各个基因的平均表达水平都有所上调,其中显著上调FGF5(P<0.0001),而对β-catenin、MSX-2、Wnt10b、BMP2、BMPRIB、NTRK3、Delta1、hairless等无显著影响(P>0.05);(2)埋植褪黑素明显提高了FGF5与hairless的相关性,并且使FGF5与β-catenin、NTRK3的相关性由负相关变成正相关,与Wnt10b的相关性降低,FGF5与其余4个基因的相关性变化不大。结论:结果提示,褪黑素上调绒山羊皮肤FGF的表达,影响FGF5与hairless、β-catenin、NTRK3之间的相关性;山羊中FGF5基因对被毛表型的作用与其他动物不同;褪黑素对复杂组织和单个细胞的作用不同;MAPK信号通路是受褪黑素影响最大的一个信号途径。推测褪黑素可能通过调节绒山羊皮肤中RORα来发挥作用,影响FGF5与hairless、β-catenin、NTRK3和Wnt10b等的相关性,同时抑制MSX2和BMP2的功能,进而促进绒毛的生长。The aim is to study the effect of melatonin on genes which related to hair growth on skin, and explore the mechanism of melatonin in cashmere growth. Using the Inner Mongolia white cashmere goats as the research object, 10 lambs on 4 months age with similar body condition were selected, and divided into 2 groups randomly. Following testing requirements, the treatment group was implanted melatonin in subcutaneous behind the ear every two months according to 2rag / kg BW （ body weight） dose, and nothing to the control. The experiment time was last for one year. Then the cashmere goat skin and hair in the body side were gathered, using real-time PCR to detect the expression changes of 9 genes related to hair growth. The results showed that （ 1 ） melatonin implanting can up-regulate FGFS＇ s expression significantly （ P 〈 0.0001 ）, but has no significantly effect to ^-eatenin, MSX-2, Wntl0b, BMP2, BMPR I B, NTRK3, Dehaland hairless （P 〉0.05）; （2） melatonin implanting obviously improved the correlation between FGF5 and hairless, and make the negative correlation turn to positive correlation on ^-eatenin and NTRK3, the correlation with WntlOb was reduced, while the other 4 were little changed. These results indicated that Melatonin up-regulate the expression of FGF5 in different. MAPK signaling pathway is the greatest impacted by melatonin. It is putative that melatonin can regulate RORot in cashmere goat skin to impact the correlation of FGF5 with hairless, 13-catenin, NTRK3 and Wntl0b, and inhibit the function of MSX2 and BMP2 for promoting hair growth.

关 键 词：褪黑素 绒山羊 FGF5 绒毛生长机理 

分 类 号：Q819[生物学—生物工程]