白细胞介素28B(IL-28B)基因多态性与丙型肝炎病毒(HCV)感染者自发清除的相关性  

Genetic variation in IL-28B and spontaneous clearance of hepatitis C virus

作　　者：丛瑞[1] 佟小非[1] 刘三都 谢勇 尤红[1] 

机构地区：[1]首都医科大学附属北京友谊医院肝病中心,北京100050 [2]贵州省黔南州人民医院感染科,贵州黔南558000 [3]贵州省平塘镇人民医院感染科,贵州黔南558300

出　　处：《首都医科大学学报》2012年第3期326-329,共4页Journal of Capital Medical University

基　　金：国家"十一五"重大科技专项(2008ZX10002-013;2009ZX1005-016)~~

摘　　要：目的本研究旨在探讨白细胞介素28B(interleukin-28B),IL-28B)基因多态性与丙型肝炎病毒(hepatitis C virus,HCV)感染者自发清除的关系。方法入组122例HCV感染者(其中24例自发清除者,98例慢性丙型肝炎患者),检测2组患者IL-28B基因周围rs12979860,rs8099917,rs10853728,rs12980275,rs4803219,rs4803223,rs8105790共7个位点的基因型。检测其中慢性丙型肝炎患者组未经抗病毒治疗时丙氨酸氨基转移酶(ALT)及HCV-RNA水平;采用非参数秩和检验比较7个位点不同基因型病毒载量水平的差异。结果自发清除组患者的平均感染年龄比慢性丙型肝炎组患者小[分别为(17.8±14.7)岁及(28.8±14.6)岁,P=0.002];rs12979860位点CC/CT、rs8099917位点TT/TG、rs10853728位点CC/CG、rs12980275位点AA/AG、rs4803219位点CC/CT、rs4803223位点AA/AG、rs8105790位点TT/TC不同基因型自发清除率分别为22.9%/7.7%(P=0.146)、22.0%/9.1%(P=0.279)、22.7%/14.9%(P=0.293)、22.7%/8.3%(P=0.196)、22.2%/8.7%(P=0.238)、16.7%/30.8%(P=0.109)、23.2%/7.4%(P=0.123)。在上述7个位点中,慢性HCV感染者HCV RNA水平在不同的IL-28B基因型间,差异无统计学意义,P值分别为0.836、0.715、0.301、0.301、0.486、0.996、0.312。结论 HCV感染者自发清除与年龄有关,感染时年龄越小,越容易清除病毒,而IL-28B基因对我国HCV感染者自发清除的影响不明显,此外IL-28B基因与慢性丙型肝炎病毒感染患者的基线HCV RNA水平无明显相关性。Objective Single nucleotide-polymorphisms （SNPs） , close to the interleukin-28 （IL-28B） gene, have been associated with spontaneous clearance of hepatitis C virus in acute hepatitis C patients in a heterogeneous population. The authors analyzed the association between IL-28B and spontaneous clearance of acute hepatitis C virus （HCV） infeetion in China. Methods A total of 122 HCV- infected persons were enrolled in the study. The rs12979860, rs8099917, rs10853728, rs12980275, rs4803219, rs4803223 and rs8105790 were detected by PCR and sequencing. They were compared between spontaneously cleared HCV and ehronically infected group, and different level of HCV RNA in chronically infected patients. Results Patients who spontaneously cleared HCV were younger than chronically infected group; spontaneous elearance rate of rs12979860 genotype CC/CT, rs8099917 genotype TT/TG, rs10853728 genotype CC/CG, rs12980275 genotype AA/AG, rs4803219 genotype CC/CT, rs4803223 genotype AA/AG, rs8105790 genotype TF/TC were not significantly different （22.9% vs 7.7% , P=0. 146; 22% vs 9. 1% , P =0.279; 22.7% vs 14.9% , P =0.293; 22.7% vs 8.3%, P=0.196; 22.2% vs8.7%, P=0.238; 16.7% vs 30.8%, P=0.109; 23.2% vs7.4%, P=0.123）. Conclusion No significant difference in IL-28B polymorphisms was found between the two groups. However, the age of infection was associated withspontaneous clearance.

关 键 词：IL-28B 丙型肝炎 基因多态性 

分 类 号：R512.63[医药卫生—内科学]