低氧诱导VEGF基因表达载体治疗家兔肢体缺血性疾病的实验研究  

Experimental study of hypoxia-inducible expression vector carrying VEGF gene on rabbit limb ischemia models

作　　者：殷爱红[1] 武文琦[1] 崔世军[2] 滕旭[3] 郑少鹏[3] 

机构地区：[1]首都医科大学医学实验与测试中心,北京100069 [2]首都医科大学宣武医院胸心血管外科,北京100053 [3]首都医科大学基础医学院生物化学与分子生物学系,北京100069

出　　处：《首都医科大学学报》2012年第3期356-360,共5页Journal of Capital Medical University

基　　金：北京市教育委员会科技发展计划面上项目(KM200310025094)~~

摘　　要：目的探讨低氧诱导血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)基因表达载体对家兔肢体缺血性模型的基因治疗效果。方法通过切除髂外动脉远端和股动脉分支制作家兔左后肢缺血模型,术后立即将含VEGF基因的低氧诱导表达载体6HRE-pcDNA-VEGF165多点注射至患侧肌肉内,并于不同时间点进行后肢胫动脉血压测量和动脉血管造影。结果与对照组相比,应用低氧诱导VEGF基因表达载体的基因治疗组胫动脉血压恢复快;动脉血管造影显示基因治疗组动脉充盈早于对照组,新生血管多于对照组,早于对照组建立侧支循环。结论低氧诱导VEGF基因表达载体可促进家兔肢体缺血模型新生血管的形成,改善缺血肢体的血液循环。Objective To investigate the gene therapy effect of hypoxia-inducible expression vector carrying VEGF gene in rabbit limb ischemia models. Methods The rabbit models of left hindlimb ischemia were developed by ligation of the distal external iliac artery and excision of branches of femoral arteries. When the rabbit models were established, the recombinant plasmids of hypoxia-indueible expression vector 6HRE-pcDNA-VEGF165 was transferred by intramuscular injection to the left hind limb immediately. At different time points, every animal was evaluated by the measurement of lower limb calf blood pressure and angiography. Results Lower limb calf blood pressure in the experimental group which was applied of hypoxia-inducible gene expression vector carrying hVEGF was recovered rapidly than in the control group. Artery angiography showed that distal arteries were inflated earlier and there were more angiogenesis in the gene therapy group than in the control group. Also the collateral circulation was established earlier in the experimental group. Conclusion Hypoxia-inducible VEGF gene expression vector could improve angiogenesis for the rabbit limb ischemia models and improve blood circulation in ischemic limbs.

关 键 词：低氧诱导表达载体 血管内皮细胞生长因子 肢体缺血 基因治疗 

分 类 号：R654.4[医药卫生—外科学] Q782[医药卫生—临床医学]