睡眠剥夺大鼠腹外侧视前区腺苷水平的变化  

Adenosine level of variation in rat ventrolateral preopic area after sleep deprivation

作　　者：江传玮[1,2] 赵乐章[1] 张瑾[1] 尹豆[1] 吴芳[1] 王烈成[1] 

机构地区：[1]安徽医科大学基础医学院生理学教研室,合肥230032 [2]安徽中医学院生理学教研室,合肥230038

出　　处：《安徽医科大学学报》2012年第7期763-767,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81071075);教育部博士点基金(编号:20093420120004);安徽省高校优秀青年人才基金(编号:2009SQRZ047)

摘　　要：目的研究睡眠剥夺大鼠促睡眠中枢腹外侧视前区(VLPO)内腺苷变化规律。方法将SD大鼠随机分为对照组和睡眠剥夺组。采用脑立体定位、微透析、多导睡眠描计技术和高效液相色谱法观察两组大鼠的睡眠剥夺前、睡眠剥夺期以及睡眠剥夺后恢复期VLPO内腺苷含量变化。结果与对照组比较,睡眠剥夺组睡眠剥夺前觉醒(W)、非快速眼动(NREM)睡眠和快速眼动(REM)睡眠无明显变化;而在睡眠剥夺期W时间增加58.5%(P<0.01),NREM时间减少55.7%(P<0.01),REM时间差异无统计学意义;在睡眠剥夺后恢复期W时间减少18.4%(P<0.05),NREM时间增加17.4%(P<0.05),REM时间差异无统计学意义。与对照组比较,睡眠剥夺开始的2 h内睡眠剥夺组的VLPO内腺苷相对含量无显著变化。而随后的4 h VLPO内腺苷相对含量明显升高(P<0.05)。睡眠剥夺后恢复期的3 h内睡眠剥夺组的VLPO内腺苷相对含量显著减少(P<0.05)。结论腺苷在VLPO内参与睡眠-觉醒周期的调节。Objective To investigate the variational regulation of adenosine （AD） in ventrolateral preopic （VL- PO） area after sleep deprivation in rats. Methods Rats were randomly divided into control group and sleep depri- vation group. Each group had 6 rats. The concentrations of AD in VLPO during baseline, sleep deprivation and sleep recovery period of two groups were studied by brain stereotaxic, microdialysis, polysomngraphy and high performance liquid chromatograph. Results Compared with the control group, the ratio of wake （W） , non- rapid eye movement （NREM） sleep and rapid eye movement （REM） sleep had no significant difference during baseline peri- od. The ratio of W was enhanced by 58.5% （P 〈0. 01 ） , NREM sleep was decreased by 55.7% （P 〈0. 01 ）, and REM sleep was no significant change during sleep deprivation period. The ratio of W was decreased by 18.4% （P 〈0. 05）, NREM sleep was enhanced by 17.4% （P 〈 0. 01 ）, and REM sleep was no significant change during sleep recovery period. Compared with the control group, the mean of AD concentration had no significant difference in initial 2 h during sleep deprivation period, however, the mean of AD concentration increased significantly during subsequent 4 h sleep deprivation period （ P 〈 0. 05 ）, and during 3 h sleep recovery period, the mean of AD concentration decreased （ P 〈 0. 05 ）. Conclusion AD in VLPO participates in the sleep-wakefulness cycle regulation.

关 键 词：腹外侧视前区 腺苷 睡眠 觉醒 

分 类 号：R338.2[医药卫生—人体生理学]