白细胞介素-17对大鼠心肌梗死后室性心律失常的影响  

Effect of IL-17 expression on ventricular arrhythmias in post-myocardial infarction rats

作　　者：陈昭喆[1,2] 陈志坚[1,2] 曾秋棠[1,2] 刘学刚[1,2] 张青[1,2] 刘立新[1,2] 朱云涛[1,2] 钱程[1,2] 杜以梅[1,2] 赵宁[1,2] 

机构地区：[1]华中科技大学协和医院心内科 [2]华中科技大学同济医学院心血管病研究所 湖北省生物靶向治疗研究重点实验室,武汉430022

出　　处：《临床心血管病杂志》2012年第6期422-426,共5页Journal of Clinical Cardiology

基　　金：国家重点基础发展研究973计划(No:2007CB512005);国家自然科学基金项目(No:30770880/30971242)

摘　　要：目的:探讨大鼠心肌梗死(MI)后白细胞介素-17(IL-17)在室性心律失常发生中的作用。方法:采用液氮冷冻法建立大鼠MI模型共138只,随机分为MI对照组(MI组)、小鼠同型IgG组(IgG组)和IL-17抗体组。每组又随机分为3个亚组:2周组、4周组及8周组。另设假手术组。假手术组和MI组分别腹腔注射0.8mlPBS溶液,并注入0.2ml空气促使液体分散到腹腔内;IgG组、IL-17抗体组分别注射小鼠同型IgG1+PBS溶液(100μg IgG1溶于0.8ml PBS,同样注入0.2ml空气)、小鼠抗人IL-17单克隆抗体+PBS溶液(100μg IL-17抗体溶于0.8ml PBS溶液,同样注入0.2ml空气)。每隔1d用药1次,连续用药5次。运用程序电刺激方法,观察各组室性心律失常的诱发率,同时用Western blot和激光共聚焦显微镜检测IL-17蛋白表达水平。结果:与假手术组比较,MI组大鼠心肌组织IL-17表达显著增加(P<0.05),室性心律失常诱发率亦明显增高(P<0.05);与MI组比较,IL-17抗体组IL-17表达显著减少(P<0.05),室性心律失常诱发率亦明显降低(P<0.05)。大鼠心肌IL-17的表达水平和室性心律失常诱发率之间呈正相关性(r=0.88,P<0.05),但IgG组与MI组比较差异无统计学意义(P>0.05)。结论:MI后大鼠心肌组织IL-17表达明显增加,大量IL-17表达与MI后室性心律失常的发生密切相关。IL-17的大量表达可能对MI后室性心率失常折返环的形成有诱导或促进作用。Objective：To investigate the effect of IL-17 expression on ventricular arrhythmias in rats with myocardial infarction（MI）. Method：Liquid nitrogen cryoinjury method was used in rats to establish MI model.The MI model rats were randomly divided into 3 groups,MI group,IgG group and IL-17 antibady group.Then each group was randomly divided into 3 sub-groups： 2 week,4 week and 8 week group.Sham group was established.The first day after operation,PBS solution were intraperitoneally injected to sham group and MI group,mouse isotype IgG1 and PBS to IgG group,monoclonal human IL-17 antibody and PBS to IL-17 antibady group,5 times every 2 days.Programmed electrical stimulus was imposed on the rat hearts to induce ventricular arrhythmia（ventricular tachycardia and ventricular fibrillation）.The evoked electrocardiograms and evoked rates were recorded.The protein expression of IL-17 was examined by Western blot and LSCM. Result：Compared to sham group,expression of IL-17 in myocardium in MI group,IgG group and IL-17 antibody group increased obviously（P〈0.05） at 2 weeks、4 weeks and 8 weeks after operation.The evoked rates of ventricular arrhythmia also increased（P〈0.05）.Compared to MI group and IgG group,expression of IL-17 in IL-17 group was lower（P〈0.05）,and the evoked rates decreased too（P〈0.05）.While,obvious differences were not found between MI group and IgG group.Statistic analysis showed that there was a positive linear correlation between expression of IL-17 and theevoked rate（r=＋0.88,P〈0.05）. Conclusion：IL-17 expression significantly increased after MI.There is a relationship between a large number of IL-17 expression and ventricular arrhythmias in rats with MI.High expression of IL-17 may induce or promote the formation of the reentrant circuit of ventricular arrhythmias in post-myocardial infarction rats.

关 键 词：心肌梗死 白细胞介素-17 室性心律失常 

分 类 号：R542.2[医药卫生—心血管疾病]