香青兰总黄酮对短暂性脑缺血诱导的炎症反应的影响  

Effect of Total Flavones of Dracocephalum moldavica L. on Inflammation Induced by Transient Cerebral Ischemia

作　　者：孙雅煊[1] 刘婷[2] 戴雪伶[3] 高兆兰[1] 郑秋生[2] 姜招峰[1,3] 

机构地区：[1]北京联合大学应用文理学院,北京100191 [2]石河子大学药学院,石河子832003 [3]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《生物物理学报》2012年第6期469-476,共8页Acta Biophysica Sinica

基　　金：北京市属高等学校人才强教计划资助项目(PHR201108422;PHR201107150)~~

摘　　要：为了研究香青兰总黄酮在大鼠短暂性脑缺血损伤中的作用及可能机制,将雄性Wistar大鼠随机分为模型组、假手术组、香青兰总黄酮高剂量(50 mg/kg)组、香青兰总黄酮中剂量(25 mg/kg)组,以及香青兰总黄酮低剂量(12.5 mg/kg)组。采用线栓法建立大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型。再灌后22 h,测定脑梗死面积、组织病理学变化、髓过氧化物酶(myeloperoxidase,MPO)活性和细胞间黏附分子-1(intercellular adhesionmolecule-1,ICAM-1)的表达。结果显示香青兰总黄酮可明显减少缺血区域梗死面积、改善组织病理学变化、降低MPO活性和ICAM-1的表达。在三个香青兰总黄酮处理组中,高剂量香青兰总黄酮作用最强。以上结果表明,香青兰总黄酮可以通过减轻炎症反应,对抗短暂性脑缺血,保护脑组织。In order to study the effect and possible mechanism of total flavones of Dracocephalum moldavica L. （TFDM） on transient cerebral ischemic injury in rats, male Wistar rats were randomly divided into five groups： the model group, the sham group, the high-dose TFDM group （50 mg/kg）, the middle-dose TFDM group （25 mg/kg）, and the low-dose TFDM group （12.5 mg/kg）. Middle cerebral artery occlusion （MCAO） model was established with the suture method. The infarction size, histopathology change, myeloperoxidase （MPO） activity and intercellular adhesion molecule-1 （ICAM-1） expression were measured at 22 h after reperfusion. The results showed that TFDM could significantly reduce the infarct size, alleviate histopathological injury, decrease the activity of MPO and extenuate ICAM-1 expression in the ischemic region. Among the three TFDM treated groups, the high-dose TFDM showed the best efficacy. The results suggested that TFDM was able to protect brains from transient cerebral ischemia by reducing the inflammatory reaction.

关 键 词：香青兰 总黄酮 短暂性脑缺血 炎症反应 

分 类 号：R285.5[医药卫生—中药学]