全蝎蜈蚣对CIA大鼠外周血CD4^+CD25^+FoxP3^+Treg细胞的调节  

Regulation Effect of Scorpio and Scolopendra on CD4^+CD25^+FoxP3^+Treg Cell in Peripheral Blood from Rats with Collagen-Induced Arthritis(CIA)

作　　者：刘端勇[1] 赵海梅[1] 黄小英 左志琴[1] 王艳辉[3] 程绍民[4] 

机构地区：[1]江西中医学院科技学院,江西南昌330025 [2]江西中医学院科研处,江西南昌330004 [3]南昌大学抚州医学分院,江西抚州334000 [4]江西中医学院基础医学院,江西南昌330004

出　　处：《中药材》2012年第4期525-528,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(30860377);江西省自然科学基金项目(2009GZY0118);江西省卫生厅科技计划(20092052)

摘　　要：目的:观察全蝎蜈蚣对胶原免疫性关节炎(Collagen-Induced Arthritis,CIA)大鼠外周血CD4+CD25+FoxP3+Treg细胞的调节作用。方法:Wistar大鼠60只,随机分成6组,分别为正常对照组、模型对照组、全蝎蜈蚣高(0.4 g/kg)、中(0.2 g/kg)、低剂量(0.1 g/kg)组和Ⅱ型胶原蛋白(60μg/kg)治疗组(CⅡ组),二型胶原蛋白诱导大鼠关节炎,并采用流式细胞术检测大鼠外周血CD4+CD25+FoxP3+Treg细胞及酶联免疫吸附试验检测血清中IL-2表达水平。结果:与模型组比较,全蝎蜈蚣高、低剂量组大鼠外周血CD4+CD25+T细胞及CD4+CD25+FoxP3+Treg细胞水平显著升高,且中、低剂量组血清IL-2水平显著下降。结论:全蝎蜈蚣治疗关节炎大鼠可能是通过上调外周CD4+CD25+FoxP3+Treg细胞表达水平以恢复或重建免疫耐受而实现的。Objective ：To study the regulation effect of Scorpio and Scolopendra on CD4＋ CD25＋ FoxP3＋ Treg Cell in peripheral blood from rats with collagen-induced arthritis. Methods：Sixty female Wistar rats were randomly divided into 6 groups, normal control group, model control group, low-dose, middle-dose and high-dose Scorpio and Scolopendra group, and the typeⅡ collagen group. Rats＇ arthritis was induced by collagen. The number of CD4＋ CD25＋ FoxP3＋ Treg cell in peripheral blood was tested by flow cytometry, and the level of IL-2 in serum was detected by enzyme linked immunosorbent assay. Results：Compared with the model groups, the levels of CD4＋ CD25＋ Treg cell and CD4 ＋ CD25＋ FoxP3＋ Treg cell were increased obviously in the high and low dose of Scorpio and Scolopendragroups （P 〈0. 05 or P 〈0.01 ） ,while the level of IL-2 in serum was decreased remarkably in the middle and low dose of Scorpio and Scolopendra groups （P 〈0.05 or P 〈0. 01 ）. Conclusion：It is realized that Scorpio and Scolopendra effectively treat RA by regulating the level of CIM＋ CD25＋ FoxP3＋ Treg cell to restore immunological tolerance.

关 键 词：全蝎 蜈蚣 类风湿性关节炎 调节性T细胞 

分 类 号：R285.5[医药卫生—中药学]