秦皮总香豆素超声辅助提取工艺优化  

Optimization on Ultrasonic Extraction Technology of Total Coumarines in Cortex Fraxini

作　　者：熊利芝[1] 马青[1] 龙华[1] 

机构地区：[1]吉首大学/植物资源保护与利用湖南省高校重点实验室,湖南吉首416000

出　　处：《中药材》2012年第4期634-637,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(51142001);湖南省教育厅优秀青年项目(11B099);湖南省科技厅科研条件创新项目(2011TT2038);返校博士科研资助经费研究项目(jsdxxcfxbskyxm201111);植物资源保护与利用湖南省高校重点实验室开放基金(JSK201108);生态旅游应用技术湖南省重点实验室开放基金(JDSTLY201112)

摘　　要：目的:优化秦皮总香豆素的超声醇提工艺。方法:采用单因素试验方法研究超声辅助提取过程中乙醇体积分数、料液比、提取温度、提取时间和超声频率对秦皮总香豆素提取率的影响,并采用正交试验确定最佳提取工艺条件。结果:秦皮总香豆素超声辅助提取的最佳提取工艺为:按1∶10(质量-体积比)的料液比加入60%乙醇,于50℃条件下用175 W超声辅助提取50 min,秦皮总香豆素提取率可达到6.283%。结论:与传统索氏提取相比,该工艺具有耗时少、耗能少和提取率高等优点。Objective：To optimize the ultrasonic extraction technology of total coumarins in Cortex Fraxini. Methods ：The effects of ethanol concentration, ratio of solid to liquid, extraction temperature, ultrasonic frequency and extraction time in extraction procedure on the extraction rate of total coumarins in Cortex Fraxini was researched by single factor experiment, the optimal extraction technology was determined by orthogonal experiment. Results ：The optimal extraction technology of total coumarins in Cortex Fraxini was：mixing the material with 60% ethanol according to ratio of solid to liquid 1：10 （W/V）, ultrasonic frequency 175 W and extracting 40 min at 50℃ . The extraction rate of total coumarins reached 6. 283% with the optimal extraction technology. Conclusion：Compares with the classic Soxhlet extraction, ultrasound is a less time-comsuming, low energy and great efficiency tool for the fast extraction of coumarinz from Cortex Fraxini.

关 键 词：秦皮 香豆素 提取 超声波辅助 

分 类 号：R284.1[医药卫生—中药学]