山东肿足蕨总三萜的提取工艺优化研究  

Optimum Extraction Technology of Total Triterpenoids from Hypodematium sinense

作　　者：张华丽[1] 朱军[1] 高桂花[1] 张敏娜[1] 李彦连[2] 

机构地区：[1]济宁医学院药学院,山东日照276826 [2]山东济宁学院生命科学与工程系,山东曲阜273155

出　　处：《中药材》2012年第4期648-650,共3页Journal of Chinese Medicinal Materials

基　　金：济宁医学院青年科研基金重点项目(JYQN)

摘　　要：目的:优化山东肿足蕨总三萜提取工艺。方法:以5%香草醛-冰醋酸溶液和72%浓硫酸为显色系统,总三萜含量为考察指标,采用单因素和正交试验优选山东肿足蕨总三萜的提取工艺。结果:确定用12倍量60%乙醇为溶剂,在60℃条件下超声提取60 min为最佳提取条件。结论:优选的工艺稳定可行,可作为山东肿足蕨总三萜的提取工艺。Objective ：To optimize the extraction technology of total triterpenoids from Hypodematium sinense. Methods：With 5% vanillin-glacial acetic acid solution and 72% sulfuric acid as chromogenic agent and the content of total tripenoids as index, using single factor experiment and orthogonal test, the optimal extraction condition was determined. Results：The optimal conditions were solid-liquid ratio 1： 12,60% ethanol concentration, and ultrasonic extraction time of 60 min at 60℃. Conclusion：The extraction technology is feasi- ble and can be used as extraction process of total triterpenoids from Hypodematium sinense.

关 键 词：山东肿足蕨 总三萜 正交试验 

分 类 号：R284.2[医药卫生—中药学]