正交法研究云南粗根荨麻多糖的提取条件  

Orthogonal design study the extraction conditions of Yunnan Urtica Macrorrhiza Hand-Mazz polysaccharide

作　　者：李仲昆 王崇静 王珩 尹为民[2] 

机构地区：[1]昆明市延安医院,650051 [2]云南省第一人民医院

出　　处：《中国实用医药》2012年第15期18-20,共3页China Practical Medicine

基　　金：云南省科技计划面上项目(项目编号:2008ZC147M)

摘　　要：目的研究提取和测定云南粗根荨麻多糖的方法。方法用正交法对云南粗根荨麻多糖的提取条件进行研究,用硫酸-苯酚比色法对多糖的含量进行测定。结果粗根荨麻水提物、粗多糖、精制多糖的多糖含量分别为12.3%、30.4%、42.7%。结论提取和测定粗根荨麻多糖的方法简便易行,稳定可靠。Objective To study the methods of extraction and determination Yunnan Urtica Macrorrhiza Hand-Mazz polysaccharide.Methods To study the extraction conditions of Yunnan Urtica Macrorrhiza Hand-Mazz polysaccharide with orthogonal design.The polysaccharide was determined by colourimetry with sulfuric acid-phenol as colourimetric resgent.Results Yunnan Urtica Macrorrhiza Hand-Mazz aqueous extracts,polysaccharide,purification of the polysaccharide contents are respectively 12.3%,30.4%,42.7%.Conclusion Extraction and determination of Yunnan Urtica Macrorrhiza Hand-Mazz polysaccharide method is simple,stable and reliable.

关 键 词：云南粗根荨麻 正交设计 多糖 

分 类 号：TQ461[化学工程—制药化工]