酸性染料比色法测定延胡索总生物碱的含量  

Determination of Total Alkaloid in Corydalis yanhusuo W.T.Wang by Acid Dye Colorimetry

作　　者：费艳美[1] 张玉萍[1] 方步武[2] 

机构地区：[1]天津中医药大学中药学院,天津300073 [2]天津医科大学,天津300070

出　　处：《现代中药研究与实践》2012年第3期66-68,共3页Research and Practice on Chinese Medicines

摘　　要：目的探讨延胡索中总生物碱含量测定的方法。方法采用酸性染料比色法测定延胡索中总生物碱含量。结果酸性染料比色法的显色条件为pH 4.4缓冲液12.0 mL,溴甲酚绿液3.0 mL,氯仿9mL萃取一次,振摇2 min,静置40 min。生物碱在1.33μg/mL～11.56μg/mL范围内呈线性关系,回收率平均值100.4%,,RSD=1.01%(n=6)。结论本含量测定方法操作简单,灵敏度高,准确度好,重现性好,可用于测定延胡索总生物碱的含量。Objective To investigate the determination method of total alkaloids in Corydalis yanhusuo W. T. Wang. Method Contents of total alkaloid were determined by Acid dye Colorimetry. Results Acid dye color con- ditions was as follows： pH4.4 buffer 12 mI., bromocresol green liquid 3.0 mL, chloroform 9.0 mL extracted one time for 2 rain, put it aside for at least 40 min. The concentration showed good linearity within the range of 1.33 μg/mL -11.56 μg/mL ,r = 1.000, recovery rate was 100.4%, RSD was 1.01% （n =6）. Conclusion The method was simple, highly sensitive and reproducible, which could be used for determination of the total alkaloids in Corydalis yanhusuo W. T. Wang .

关 键 词：酸性染料比色法 延胡索 总生物碱 

分 类 号：R927.2[医药卫生—药学]