RP-HPLC和UV-VIS法测定灵芝不同收获期的多糖肽和灵芝酸  

Determination of polysaccharide peptides and ganoderic acids of Ganoderma Lucidum in different harvest periods by RP-HPLC and UV-VIS methods

作　　者：王赛贞[1,2] 林冬梅[1] 林占熺[1] 林志彬[3] 林树钱[1,2] 罗虹建[1] 

机构地区：[1]福建农林大学菌草研究所,福建福州350002 [2]福州绿谷生物药业技术研究所,福建福州350002 [3]北京大学医学部药理系,北京100083

出　　处：《药物评价研究》2012年第3期190-193,共4页Drug Evaluation Research

摘　　要：目的研究灵芝不同栽培方式和不同收获期中多糖肽和灵芝酸A、B的变化,为灵芝规范化栽培及制定产品质量标准提供依据。方法 RP-HPLC测定菌草灵芝和仿野生栽培的灵芝提取物的灵芝酸A、B的变化。色谱柱为XB-C18柱(150mm×4.6mm,5μm),流动相为0.05%磷酸-乙腈(65:35),检测波长254nm,常温,进样量50μL。以灵芝多糖肽为对照品,采用Folin-酚试剂法,通过紫外-可见吸收光谱法检测菌草灵芝和仿野生栽培灵芝不同收获期提取物的多糖肽。结果灵芝酸A、B线性范围分别为14.8～371.0和16.4～409.0μg/mL,平均回收率为95.0%、100.5%,RSD分别为1.18%、3.37%(n=6)。灵芝多糖肽在100～600μg/mL与吸光度值呈线性关系,r=0.9995,回收率95%～100%。结论该方法简便、快速,可用于灵芝及其产品中多糖肽和灵芝酸A、B的质量控制。菌草灵芝沸水提取能将多糖肽和灵芝酸分离,既简化了灵芝水、醇提取工艺,又提高经济效益。Objective To study the changes of Ganoderma lucidum polysaccharide peptides （GLPP） and ganoderic acids A and B by different cultivation methods, which provides the basis for G. lucidum standardized cultivation and establishment of its product quality standard. Methods To determine the changes of ganoderic acids A and B between grass-cultivated G. Lucidum （GCGL） and wood logs cultivated G. lucidum （WLCGL） extracts by RP-HPLC method. Chromatography on XB-C18 column （150 mm × 4.6 mm, 5μm） was used and the mobile phase was 0.05% phosphoric acid-acetonitrile （65：35） with detection wavelength of 254 nm at ambient temperatures and sample quantity of 50μL. With GLPP as reference, the content of polysaccharide peptide from GCGL and WLCGL extracts was tested in UV-VIS by using the method of Folin-phenol reagent. Results Linear ranges ofganoderic acids A and B were 14.8-371.0 and 16.4 409.0 μg/mL, respectively. The average recovery rate ofganoderic acids A and B were 95.0% and 100.5%, and RSD were 1.18% and 3.37% （n = 6）, respectively. In the range of 100-600 μg/mL, linear relationship was between concentrations and absorbance of GLPP, r = 0.999 5, with the recovery rate of 95%-100%. Conclusion The method is simple and convenient, which could be used for the quality control of GLPP and the changes of ganoderic acid A and B. It is found that GCGL could be extracted from boiling water, which could separate GLPP and ganoderic acids at the same time. It could not only simplify the alcohol and water extraction process of G. lucidum, but also improve the economic efficiency.

关 键 词：菌草灵芝 仿野生栽培灵芝 灵芝多糖肽 灵芝酸A 灵芝酸B RP—HPLC 

分 类 号：R282.6[医药卫生—中药学]