季节性H1N1流感病毒对Hep-2细胞Toll样受体表达的影响  

Effect of influenza virus A (H1N1) on Toll-like receptors expression in Hep-2 cells

作　　者：韩焱[1] 任月[1] 徐明玉[1] 孙楠[1] 

机构地区：[1]大连市疾病预防控制中心,辽宁大连116021

出　　处：《疾病监测与控制》2012年第6期321-322,共2页Journal of Diseases Monitor and Control

基　　金：大连市卫生局资助项目

摘　　要：目的观察TLRmRNA在季节性H1N1流感病毒感染的人喉癌Hep-2细胞中动态表达,为进一步研究流感病毒对机体免疫反应的影响提供依据。方法采用Real-timePCR对季节性H1N1流感病毒感染不同时间Hep-2细胞中TLR3,TLR7,TLR8和TLR9的mRNA表达量进行检测。结果流感病毒感染Hep-2细胞48h后,所有被检测的TLRmRNA表达量都有不同程度上升,其中TLR8和TLR9的mRNA提高显著分别是对照组的13.9和14.3倍。结论推测TLR8和TLR9在季节性H1N1流感病毒诱导Hep-2细胞的免疫反应过程中起重要作用。Objective To observe the dynamic expression of mRNA of TLR in Hep-2 cells after exposure to influenza virus A （H1N1）. Methods We infected Hep-2 cells with H IN1 for different periods of time, and then assessed expression of TLR3, 7, 8, 9 by the real-time reverse-transcriptase polymerase chain reaction. Results ARcr HINI infected for 48 h, all the detected TLR mRNA levels were upregulated, especially TLR8 and TLR9 which were increased 13.9 and 14.3-fold over the uninfected ceils, respectively. Conclusion TLRS and TLR9 may be involved in influenza virus-induced irnmunc response in Hep-2 cells.

关 键 词：流感病毒 TOLL样受体 REAL-TIMEPCR 

分 类 号：R511.7[医药卫生—内科学]