2株菌混合降解拟除虫菊酯类农药条件的优化及其协同作用  

Studies on degradation characteristics of pyrethroid insecticides by one mixed strain and its synergistic effect

作　　者：罗建军[1] 陈少华[1] 胡美英[1] 陈慧婷[1] 张芳[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广东广州510642

出　　处：《西北农林科技大学学报（自然科学版）》2012年第6期125-132,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30871660)

摘　　要：【目的】制备高效混合菌,为控制拟除虫菊酯类农药残留提供候选生物制剂。【方法】以蜡状芽孢杆菌(Bacillus cereus)ZH-3和金色链霉菌(Streptomyces aureus)HP-S-01混合菌为材料,采用单因素试验优化其生长和降解条件,高效液相色谱法(HPLC)测定其降解能力。【结果】混合菌生长和降解拟除虫菊酯类农药的最优条件为接种量0.4g/L、28℃、pH 7.5、振荡速率150r/min,在此条件下培养72h后,混合菌对50mg/L氯氰菊酯的降解率达90%以上。混合菌高度耐受并降解氯氰菊酯,在氯氰菊酯初始质量浓度为100～500mg/L时,降解率达到80%以上。混合菌最佳接种比例为1∶1,培养72h后该比例混合菌对50mg/L氯氰菊酯、氰戊菊酯和联苯菊酯的降解率分别为91.6%,92.5%和95.7%,比单一菌ZH-3和HP-S-01的降解率均显著提高。【结论】混合菌对3种拟除虫菊酯类农药的降解存在协同增效作用。【Objective】 The study aimed to prepare the efficient mixed strain,and to provide promising candidate biological agents for controlling pyrethroid-contaminated environments.【Method】 The mixed strain were composed upon two isolated strains Bacillus cereus ZH-3 and Streptomyces aureus HP-S-01.The optimal growth and degradation conditions were determined through single-factor experiments.The degradation ability of the mixed strain was evaluated with high performance liquid chromatography（HPLC）.【Result】 The optimal conditions for growth and biodegradation were obtained as follows：inoculum biomass 0.4 g/L,temperature 28 ℃,pH 7.5,and with shaking speed 150 r/min.Under these conditions,the mixed strain effectively degraded cypermethrin（50 mg/L） with a degradation rate up to 90% within 72 h.Furthermore,the mixed strain tolerated and efficiently degraded high concentrations of cypermethrin（100-500 mg/L） with a degradation rate up to 80% within 72 h.The optimal inoculating rate was 1∶1 based on single strain ZH-3 and HP-S-01.At such inoculating rate,the degradation rates for cypermethrin,fenvalerate and bifenthrin were 91.6%,92.5% and 95.7%,respectively.The degradation efficiency was significantly enhanced compared with the single strain.【Conclusion】 The mixed strain may have synergistic effect on degradation of cypermethrin,fenvalerate and bifenthrin.

关 键 词：拟除虫菊酯类农药 生物降解 混合菌 协同作用 

分 类 号：X592[环境科学与工程—环境工程]