青海北山林场桦树根围芽胞杆菌分子鉴定及其拮抗活性分析  

Molecular identification of Bacillus strains isolated from rhizosphere of Betula platyphylla in Qinghai Beishan timberland and its antagonistic activity analysis

作　　者：谢永丽[1,2] 徐志伟 马莉贞[2] 高学文[1] 

机构地区：[1]南京农业大学植物保护学院,农作物生物灾害综合治理农业部重点实验室,南京210095 [2]青海大学农牧学院,高原草地资源与生态省部共建重点实验室,西宁810016

出　　处：《植物保护学报》2012年第3期246-252,共7页Journal of Plant Protection

基　　金：青海省(应用)基础研究计划项目(2011-Z-725);国际科技合作项目(2009DFA32740)

摘　　要：自青海北山林场桦树根围分离筛选到芽胞杆菌菌株166株,根据菌落特征分为6个类群,选择其中31株菌株纯化保存并进行BOX-PCR及ERIC-PCR指纹图谱分析、16S rDNA及gyrB基因序列分析,鉴定出球形赖氨酸芽胞杆菌Lysinibacillus sphaericus 12株、解淀粉芽胞杆菌Bacillus amyloliq-uefaciens 5株、韦氏芽胞杆菌Bacillus weihenstephanensis 7株、芽胞杆菌Bacillus sp.5株、苏云金芽胞杆菌Bacillus thuringiensis 2株。通过平板对峙试验筛选得到11株对油菜菌核病原真菌Sclerotiniasclerotiorum具有显著拮抗活性的菌株。以MALDI-TOF-MS质谱分析拮抗菌株QB11的活性成分,可产生脂肽化合物表面活性素和泛革素,表明其拮抗活性与脂肽化合物的合成和分泌有关。One hundred and Sixty-six Bacillus strains were isolated from rhizosphere of Betula platyphylla Suk. in Beishan timberland and were classified into six groups after primary morphological identification. Thirty-one Bacillus isolates were chosen from six groups for polyphasic taxonomy identification including BOX-PCR and ERIC-PCR fingerprints analysis, as well as 16S rDNA and gyrB partial sequence analysis. The results showed that Thirty-one isolates were identified as Lysinibacillus sphaericus （Twelve isolates）, Bacillus amyloliquefaciens （five isolates ）, Bacillus weihenstephanensis （seven isolates ）, Bacillus sp. （five isolates） and Bacillus thuringiensis （two isolates）. Eleven Bacillus isolates were screened which present high antagonistic activity to Sclerotinia sclerotiorum by means of the dual culture method screen- ing. The lipopeptide compound analysis of MALDI-TOF-MS showed that strain QB11 （B. amyloliquefa- ciens） present distinct antagonistic activity to S. sclerotiorum, produced surfactin and fengycin, indicated that the bio-control efficacy of strains were related to the antifungal lipopeptide compounds produced by strains themselves.

关 键 词：芽胞杆菌 分子鉴定 拮抗活性分析 

分 类 号：S476[农业科学—农业昆虫与害虫防治]