重构肠道菌群Ⅱ型糖尿病大鼠造模血脂代谢变化及肝细胞线粒体超微结构观察  

The observations of the ultra-micstructural changes in mitochondria of hepatic cells and bloodlipid metabolism in streptozotocin-induced type-2 diabetic rats remodeled intestinal flora

作　　者：陈增强[1] 潘亮亮[2] 姜爱英[2] 陶祝华 周燕[2] 曾爱兵[2] 

机构地区：[1]温州医学院附属第一医院检验科,浙江温州325000 [2]温州医学院生命科学学院,浙江温州325035 [3]温州职工中等卫生学校,浙江温州325000

出　　处：《中国微生态学杂志》2012年第6期490-492,496,共4页Chinese Journal of Microecology

基　　金：浙江省自然科学基金(Y2100321);浙江省自然科学基金(Y3110050);温州市科技局基金项目(Y20080139)

摘　　要：目的重构肠道菌群Ⅱ型糖尿病大鼠模型脂类代谢变化比较,探讨肠道菌群潜在的调脂作用。方法将64只SD雄性大鼠随机分为对照组、造模组、益生组和去污组。采用高糖高脂喂养,亚致病剂量(30 mg/mL)链脲佐菌素腹腔一次性注射造模,分别于2周、4周后取腹腔主动脉血做血脂检测,同时处死大鼠取肝脏做电镜切片观察。结果注射4周后各组出现的胆固醇升高两两比较差异有统计学意义(P<0.01),LDL升高差异有统计学意义(P<0.01);益生组肝细胞线粒体受损明显;去污组血脂升高相对较轻,肝细胞内有大量脂肪堆积。结论益生菌对Ⅱ型糖尿病大鼠胆固醇升高有一定改善作用,肝线粒体损伤较明显;去污染组损伤相对较轻,而脂肪堆积明显;提示糖尿病脂类代谢与肠道菌群密切相关。Objective To investigate the reconstruction of the intestinal flora and the influence on the lipid metabolism of type II diabetic rats. Method 64 male SD rats were randomly divided into four groups, including control group, type-2-di- abetic-modeling group, probiotic group and depollutional group. All groups were fed with high-fat diet and inoculated streptozotoein （30 mg/mL） by intraperitoneal injection at one time except control group. We detected the bloodlipid after 2 and 4 weeks, meanwhile, observed the hepatic cells with eletronic microscopy. Result Rats＇ Cholesterol and LDL decreased signif- icantly comparing with type II-diabetic-modeling group 4 weeks after streptozotocin injection; there were significant differences among those three groups （ P 〈 0.01 ）. In the depollutional group, the bloodlipid increase was relatively less and there were lots of fat accumulated in the hepatic cells. Mitochondria of hepatic cells were significantly impaired in probiotic group, which was more serious than in depollutional group. Conclusion Remodeling intestinal flora caused ultrastructural changes in mitochondria in the hepatic cells of streptozotocin-induced type-2 diabetic rats, suggesting that intestinal flora is closely related to the lipid metabolism of type II diabetic rat.

关 键 词：糖尿病 肠道菌群 脂类代谢 线粒体 电镜 

分 类 号：R587.1[医药卫生—内分泌]