靛玉红、蛇床子素抗外阴阴道念珠菌病混合菌生物膜作用的研究  

The effects of indirubin and Osthol against mixed biofilms of vulvovaginal candidiasis

作　　者：解光艳[1] 程惠娟[1] 汪长中[1] 胡跃龙[1] 朱玲玲[1] 孙振新[1] 李露天[1] 

机构地区：[1]安徽中医学院,安徽合肥230038

出　　处：《中国微生态学杂志》2012年第6期493-496,共4页Chinese Journal of Microecology

基　　金：国家自然基金项目(81173629);安徽省教育厅自然基金项目(KJ2007B113)

摘　　要：目的研究中药有效成分靛玉红、蛇床子素抗外阴阴道念珠菌病混合菌生物膜的作用。方法体外建立白念珠菌(Candida albicans)铜绿假单胞菌(Pseudomonas aeruginosa,P.a)混合菌生物膜(Biofilm,BF),XTT减低法及形态学观察白念珠菌混合菌生物膜的形成过程;形态学观察、活菌计数法评价中药有效成分靛玉红(indirubin)、蛇床子素(Ostho)对白念珠菌混合菌生物膜的最小抑膜浓度(SMIC),并经扫描电镜确认。结果白念珠菌混合菌48 h能形成成熟的生物膜;62.5 mg/L浓度的靛玉红能抑制白念珠菌混合菌生物膜的形成。500 mg/L浓度的蛇床子素未见有抑制白念珠菌混合菌生物膜的作用。结论靛玉红由于具有抗生物膜的作用,可用于预防外阴阴道念珠菌病的复发。Objective To investigate the effects of Chinese Herbal Drugs indirubin and Osthol against mixed biofilms of vulvovaginal candidiasis. Method C. albicans and P. aeruginosa were mixed, then the mixed biofilms were developed in vitro. XTT reduction assay and morphological observation were performed to learn the formation process of C. albicans mixed biofilms. Morphological observation and CFU counting were employed to evaluate the sessile minimal inhibitory concentrations （SMICs） of indirubin and Osthol on C. albicans mixed biofilms biomass and confirmed by SEM. Result Mature mixed bio- films of C. albicans and P. aeruginosa were developed after 48 h in vitro. SMICs of indirubin and Osthol against C. albicans mixed biofilms biomass were 62.50 mg/L and 〉 500 mg/L, respectively. Conclusion Indirubin displayed potential to inhib- it mixed biofilms of vulvovaginal candidiasis, thus can be used to prevent the recurrence of vulvovaginal candidiasis.

关 键 词：靛玉红 蛇床子素 外阴阴道念珠菌病 生物膜 

分 类 号：R379.4[医药卫生—病原生物学]