化妆品中喹诺酮类和异喹啉类抗生素的高效液相色谱测定法  

Determination of Quinolones and ISO Quinolines of Antibiotics in Cosmetics by HPLC

作　　者：杨艳伟[1] 朱会卷[1] 朱英[1] 张卫强[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境与健康杂志》2012年第6期544-546,共3页Journal of Environment and Health

摘　　要：目的建立同时测定化妆品中10种抗生素的高效液相色谱(HPLC)法。方法在C1(84.6 mm×250 mm,5μm)色谱柱上,以乙腈-0.05 mol/L磷酸二氢铵溶液为流动相进行梯度洗脱,流量为1.2 ml/min,检测波长为280 nm,柱温为25℃下进行HPLC检验。结果在2～100 mg/L的线性范围内,所得10种抗生素的回归方程均呈较好的线性关系,r≥0.999 5。该方法的检出限为0.05～0.1 mg/L,RSD<3.80%,平均回收率为90.5%～104.8%。结论该方法操作简便快速,灵敏度高,适用于化妆品中10种抗生素的同时测定。Objective To establish a method for simultaneous determination of 10 kinds of antibiotics in cosmetic by high performance liquid chromatography. Methods Ten kinds of antibiotics were separated on a C18 column using acetonitrile-0.05 mol/L ammonium dihydrogen phosphate solution as mobile phase at a flow rate of 1.2 ml/min,with the column temperature 25℃ and detection wave 280 nm. Results There was a good linear relationships between the concentration of antibiotics and peak area in the rang of 2-100 mg/L. The limits of detection were 0.05-0.1 mg/L. The RSDs were less than 3.80% and the rates of recovery were 90.5%-104.8%. Conclusion This method is simple,fast,accurate and is applicable to simultaneous determination of 10 kinds of antibiotics in cosmetics.

关 键 词：化妆品 抗生素 禁用组分 色谱法 高效液相 

分 类 号：O657.6[理学—分析化学]