一测多评法测定银黄制剂中4种黄酮类成分含量  

Simultaneous Determination of Four Flavones in Yinhuang Preparations by QAMS

作　　者：杨菲[1,2,3] 冯伟红[1,2] 王智民[1,2] 张启伟[1,2] 李东影[1,2,3] 吉丽娜[1,2,3] 程翼宇[3] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中药质量控制技术国家工程实验室,北京100700 [3]天津中医药大学,天津300193

出　　处：《中国药学杂志》2012年第12期984-989,共6页Chinese Pharmaceutical Journal

基　　金：中医药行业科技专业资助项目(200707009);国家"重大新药创制"科技重大专项(2009ZX09301-005;2009ZX09308-003)

摘　　要：目的建立一测多评法测定银黄制剂中黄芩黄酮类成分含量的分析方法,证明一测多评法用于复方制剂的可行性。方法以银黄制剂为研究对象,建立汉黄芩苷(wogonoside)、黄芩素(baicalein)和汉黄芩素(wogonin)与黄芩苷(baicalin)间的相对校正因子。分别采用外标法和一测多评法测定银黄制剂中4种黄酮类成分的含量,并将一测多评法的计算值与外标法实测值用相对误差进行比较。结果黄酮类成分间的相对校正因子分别为f2汉74黄n芩m素/黄芩苷=1.20,f2黄74芩n素m/黄芩苷=1.62,f2汉74黄n芩m素/黄芩苷=1.68。一测多评法的计算结果与外标法的实测值之间没有显著性差异,实验所得的相对校正因子可信。结论一测多评法可作为一个新的质量评价模式用于银黄制剂中黄酮类成分的含量测定。OBJECTIVE To establish a quality evaluation method, quantitative analysis of multi-components with a single-marker （QAMS） , to simultaneously determine the contents of four flavones in Yinhuang preparations. METHODS Baicalin was used as the internal reference substance. The relative correlation factors （RCF） of wogonoside, baiclalein and wogonin to baiealin were calculated and established. The contents of these four flavones were determined by the＇ external Standard method and QAMS respectively. The QAMS method was validated through comparison of the results obtained by the two different methods. RESULTS Within the linear ranges, the values of RCF determined at 274 nm of wogonoside, baicalein and wogonin to baiealin were 1.20, 1.62 and 1.68 respec- tively. The RCF showed good reproducibility within the range of 1.7% -4. 8% for the three studied compounds. The two methods did not show significant difference in assay results. CONCLUSION The QAMS method is feasible leredible, and can be used to deter- mine multiple flavones in Yinhuang preparations.

关 键 词：一测多评法(QAMS) 银黄制剂 黄酮 相对校正因子 黄芩苷 黄芩素 汉黄芩素 汉黄芩苷 

分 类 号：R927[医药卫生—药学]