同步荧光光谱法测定水中痕量萘和菲  

A Method for Determination of Trace Naphthalene and Phenanthrene in Water Using Constant-Wavelength Synchronous Fluorescence Spectrometry

作　　者：宋广清[1,2] 席宏波[1] 周岳溪[1] 李杰[2] 赵京田[3] 崔俊华[3] 

机构地区：[1]中国环境科学研究院水污染控制技术研究中心,北京100012 [2]兰州交通大学环境与市政工程学院,甘肃兰州730070 [3]河北工程大学城市建设学院,河北邯郸056038

出　　处：《光谱学与光谱分析》2012年第7期1838-1841,共4页Spectroscopy and Spectral Analysis

基　　金：国家水体污染控制与治理科技重大专项项目(2008ZX07207-004)资助

摘　　要：同步荧光法具有选择性好、灵敏度高、干扰少等特点,可用于多组分多环芳烃混合物的同时测定,本文建立了恒定波长同步荧光光谱法同时测定水中萘和菲的新方法。研究了萘和菲在不同溶剂中的荧光光谱特性,确定了同步荧光的最优波长差。当Δλ=100nm,萘和菲激发波长(λex)分别为220.2和248.8nm时,在0.5～25.0μg.L-1浓度范围内,荧光强度与浓度呈现良好的线性关系,相关系数分别为0.999 5和0.999 7;萘和菲检出限均低于0.03μg.L-1,回收率在98.0%～101.5%。该方法方便快捷,预处理简单,可用于水中萘、菲的快速测定。In view of synchronous fluorescence possessing the character of good selectivity, high sensitivity, less interference, etc. it can be used for simultaneous determination of multi-component mixtures of polycyclic aromatic hydrocarbons（PAHs）. A new method of constant-wavelength synchronous fluorescence spectrometry to determine two naphthalene and phenanthrene of PAHs was developed in this study. The effect of different experimental conditions, such as different disolvents for character of fluorescence spectra and the choose of the optimal wavelength difference were studied. Experiment showed that the simultaneous indentification and quantitative determination of the two PAHs when A2= 100 was chosen. The fluorescence intensity was linearly related to naphthalene and phenanthrene concentration in the range of 0. 5-25.0 μg · L^-1 with correlation coefficient 0. 999 5 and 0. 999 7, respectively. The detection limits were all lower than 0.03μg · L-1 , and the relative standard deviations for naph- thalene and phenanthrene were 1.19% and 180%（n=7）, respectively. Results show that the compounds can be analyzed quali- tatively and quantitatively by synchronous fluorescence spectrometry.

关 键 词：同步荧光 溶剂效应 发射波长 萘 菲 

分 类 号：X52[环境科学与工程—环境工程]