罗格列酮对OLETF大鼠肺组织转化生长因子β_1及其信号转导分子的影响  

Effects of Rosiglitazone on Transforming Growth Factor-β_1 and its Signaling Pathway Genes Expressions in Pulmonary Tissue of Otsuka Long-Evans Tokushima Fatty Rats

作　　者：刘佳[1] 赵伟[1] 宋旭臣[1] 郝久营[1] 张雅静[1] 张宏[1] 

机构地区：[1]天津医科大学代谢病医院,卫生部及天津市激素与发育重点实验室,300070

出　　处：《中国慢性病预防与控制》2012年第3期271-273,236,共3页Chinese Journal of Prevention and Control of Chronic Diseases

基　　金：天津市高等学校科技发展基金项目(20080131)

摘　　要：目的探讨罗格列酮对自发性糖尿病OLETF(Otsuka Long-Evans Tokushima Fatty)大鼠肺组织纤维化的影响及其机制。方法 30周龄雄性OLETF大鼠16只,随机分为糖尿病组(DM组)和罗格列酮组(RGT组,予罗格列酮3mg·kg-1·d-1灌胃12周),对照组为LETO(Long Evans Tokushima Otsuka)大鼠8只。HE、Masson染色观察各组大鼠肺组织结构及其胶原沉积变化,免疫印迹(Westernblotting)方法检测肺组织Smad3、Smad7和Ⅲ型胶原表达,ELISA检测肺组织转化生长因子β(1transforming growth factor-β1,TGF-β1)的表达。结果与对照组大鼠相比,DM组大鼠肺组织TGF-β1、Smad3和Ⅲ型胶原表达增加,Smad7表达减少,差异均有统计学意义(P<0.05),肺组织胶原沉积增加,呈纤维化趋势;罗格列酮治疗可使RGT组大鼠上述指标逆转。结论罗格列酮对OLETF大鼠肺组织有抗纤维化作用,可能通过下调TGF-β1/Smad信号转导通路发挥作用。Objective To explore effects and its mechanism of Rosiglitazone treatment on transforming growth factor-β1/ Smad （TGF-β1/Smad） signaling pathways in Otsuka Long-Evans Tokushima Fatty （OLETF） rats. Methods Sixteen OLETF rats were divided into two groups ： DM group and rosiglitazone-treated group （RGT group）, and 8 of Long Evans Tokushima Otsuka （LETO） rats as control （N group ）. RGT group rats were treated with rosiglitazone （3 mg/kg/day, in drinking water） for 12 weeks, starting at the age of 30 weeks. The structural and collagen deposition changes of pulmonary tissue in three groups rats were observed by HE and Masson staining; the expression of Smad7 and collagen type IIl in lung tissues were examined by western blotting; the expression of TGF-β1of lung tissues were examined by ELISA. Results The expression of TGF-β1, Smad3 and collagen type m increased in pulmonary tissues of OLETF rats, which higher than those of LETO rats（P〈0.05 ）. The expression of Smad7 decreased in OLETF group（P〈0.05 ）. Pulmonary interstitial and perivascular of LETO rats had some collagen fibers, which increased and disordered in OLETF rats. Rosiglitazone partially reversed all of these parameters （P〈0.05）. Conclusions Rosiglitazone may improve diabetic pulmonary fibrosis probably via TGF-β1/Smad signaling pathways.

关 键 词：糖尿病 肺 转化生长因子β1 SMAD蛋白质类 罗格列酮 

分 类 号：R587.1[医药卫生—内分泌]