血红鸡爪槭叶片WD_(40)转录因子的克隆及序列分析  

Cloning and Sequence Analysis of WD_(40) Transcriptional Regulator of Acer palmatum

作　　者：安龙杰[1,2] 冯大领[2] 史宝胜[2] 

机构地区：[1]林木育种国家工程实验室,北京100000 [2]河北农业大学园林与旅游学院,河北保定071000

出　　处：《北方园艺》2012年第12期148-152,共5页Northern Horticulture

基　　金：林木育种国家工程实验室开放课题资助项目(FOP2010-5)

摘　　要：以血鸡爪槭叶片总RNA为模板,采用RT-PCR和RACE-PCR的方法,研究血红鸡爪槭叶片花色素苷的合成机理,成功获得了调控花色素苷表达的转录因子WD_(40)基因。结果表明:WD_(40)基因全长1 104 bp,编码337个氨基酸。通过NCBI中的Blast软件对其进行核苷酸序列和氨基酸序列的同源性进行分析,表明所获得的核苷酸序列和推导的氨基酸序列与其它物种的转录因子WD_(40)有较高的同源性,并且C端含有4个WD重复基序,推断该基因为血红鸡爪槭的WD_(40)转录因子。With Acer palmatum as materials,total RNA was isolated from the leaf of Acer palmatum and acted as template for reverse transcription polymerase chain reaction（RT-PCR）.The results showed that the full-length cDNA encoding a transcriptional factor was cloned via RACE-PCR method and the cDNA contained an ORF of 1 104 bp encoding 337 amino acids.Through Blast software in NCBI,the nucleotide sequence and the amino acid sequence of the leaf of Acer palmatum were analyzed.The results showed that the nucleotide and amino acid sequences of the cDNA in Acer palmatum were highly homologous with those of the WD40 transcription factor in other species,and four conserved WD repeat motif were presented in the C-terminal of amino acid sequences,which suggested the sequence was WD40 transcription factor gene.

关 键 词：血红鸡爪槭 WD40转录因子 基因克隆 序列分析 

分 类 号：S792.35[农业科学—林木遗传育种]