新疆南疆梨树叶表面敌敌畏降解菌的分离及特性研究  

Isolation and Degrading Characteristic of Bacteria from Pear Leaf Surfaces in Southern Xinjiang

作　　者：杨瑞红[1] 王海燕[1] 孙霞[2] 施宠[2] 

机构地区：[1]新疆教育学院理学分院,新疆乌鲁木齐830043 [2]新疆农业大学,新疆乌鲁木齐830052

出　　处：《北方园艺》2012年第12期158-160,共3页Northern Horticulture

基　　金：新疆自治区高校科研计划资助项目(XJEDU2010S17):土壤学自治区重点学科资助项目

摘　　要：从南疆长期使用有机磷农药的梨园采集叶片,分离出3株具有降解敌敌畏(DDVP)能力的细菌。经过16S rDNA的鉴定均为假单孢菌,进一步研了PP-Y1对DDVP的最佳降解条件。结果表明:PP-Y1在pH 6.5、培养7 d,DDVP降解率达53.4%;26～35℃对降解DDVP的影响不大;降解DDVP的为胞内酶。将PP-Y1随机接种到梨树叶表面和果实表面,10 d后再分离到该菌的几率为87%和79%,说明该菌较容易定殖于梨叶片和果实表面,为开发可应用的有机磷降解微生物提供资源和支持。3 bacteria capable of degrading DDVP were isolated from the pear leaves,which collected from the pear orchards with long - term application of the organophosphorus pesticides in Southern Xinjiang.All the three isolates were identificated to be Pseudomonas sp.with the help of 16S rDNA.The optimal conditions for degrading DDVP of the strain PP-Yl were as pH 6.0 and 7 days,as the degradation rate reached to 53.4%.There was no significant differences to degradation rate at 26～35℃.The intracellular enzymatic could degrade DDVP.The strain PP-Yl was used for inoculating pear leaves and fruits,and re-isolated rates were 87%and 79%at day 10,respectively.The above results suggested the strain PP-Y1 could easily colonized on the surface of pear leaves and fruits,and it provided the resources and surports for developing and applicating the degrading DDVP microbial.

关 键 词：生物降解 敌敌畏(DDVP) 假单孢菌 

分 类 号：S661.2[农业科学—果树学]