辣椒疫霉菌复壮与保存及生长特性研究  

Study on Rejuvenation,Preservation Methods and Growth Characteristics of Three Physiological Races of Phytophthora capsici

作　　者：蒋兰君[1] 巩振辉[1] 赵倩[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100

出　　处：《北方园艺》2012年第12期161-164,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(30771467);"十二五"农村领域国家科技计划资助项目(2011BAD12B00);教育部高校博士点基金资助项目(200807120007)

摘　　要：研究了辣椒疫霉菌3个生理小种的孢子囊诱导、回接复壮、短期固体培养基保存方法和复壮、培养基厚度、菌龄、生理小种对菌落生长及其形态的影响。结果表明:打菌饼诱孢较整皿光照诱孢速度快、产孢量大;发病辣椒茎段较发病叶片回接效率高,达到50%;疫霉菌在16℃下固体培养基上保存效果最好,保存时间为3～4个月;辣椒疫霉菌复壮前后Ph1、Ph3生理小种菌丝生长速率无显著差异,Ph2有显著差异,复壮后3个生理小种菌丝都变致密,致病性增强;培养基厚度可影响菌丝生长速率,10～15 mL体积的培养基较适合疫霉菌的培养;疫霉菌菌丝的菌龄对菌丝生长速率无影响;Ph1、Ph2、Ph3随着致病性的增强菌丝生长速率下降。Researches on methods of sporangium inducing,rejuvenation,preservation and growth characteristics of Phytophthora capsici were carried out.The results showed that the sporangium on mycelial plugs were more and appeared earlier than the mycelium picking out from the whole colony under light;The efficiency of stem segments’ back inoculation was higher than that of the leaf segments;The preservation effect on PSA medium under 16℃was best and preservation time was three to four months;Growth rate of Ph1 and Ph3 strains except Ph2 had no significant differences pre-and post-rejuvenation but the colony of three physiological races became compact and pathogenicity enhanced after rejuvenation;The thickness of medium had effect on the growth of colony and the best volume was 10～15 mL;The cell age of mycelium had no effect on the growth of colony;The growth rate of three physiological races slowed as their pathogenicity enhanced.

关 键 词：辣椒疫霉菌 生理小种 回接 复壮 保存 生长速率 

分 类 号：S436.418.19[农业科学—农业昆虫与害虫防治]