Cd^(2+)浓度、温度及交互作用对3株微藻生长及叶绿素荧光特性的影响  

THE INTERACTION EFFECTS OF DIFFERENT CD^(2+) CONCENTRATIONS AND TEMPERATURE ON THE GROWTH AND CHLOROPHYLL FLUORESCENCE OF 3 MICROALGAL STRAINS

作　　者：王帅[1,2] 梁英[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061

出　　处：《海洋湖沼通报》2012年第2期44-58,共15页Transactions of Oceanology and Limnology

基　　金："十一五"国家科技支撑计划(2006BAD09A03)资助

摘　　要：本文研究了不同Cd2+浓度与不同温度交互作用对中国海洋大学微藻种质库(MACC)保存的小球藻(Chlorellasp.)、微绿球藻(Nannochloris oculata)和杜氏盐藻(Dunaliella salina)的叶绿素荧光参数(PSII的最大光化学量子产量Fv/Fm、PSII的实际光能转化效率Yield、光化学淬灭qP和非光化学淬灭NPQ)、叶绿素相对含量以及细胞密度的影响。结果表明,随着Cd2+浓度的升高,3株微藻的主要荧光参数、叶绿素相对含量和细胞密度均呈明显下降趋势。但微绿球藻的荧光参数Fv/Fm在对照组与10μmol/LCd2+浓度时基本一致,这可能与低浓度Cd2+在一定程度上刺激了微藻生长有关。相关性分析结果表明,3株微藻荧光参数Fv/Fm和Yield均与Cd2+浓度呈极显著的负相关,小球藻和微绿球藻的叶绿素相对含量和细胞密度与Cd2+浓度呈显著的负相关,杜氏盐藻的叶绿素相对含量和细胞密度在48h后与Cd2+浓度呈显著的负相关,随着Cd2+浓度的增加而降低。多重比较结果表明,小球藻在25℃时对Cd2+的耐受性最强,该温度下荧光参数(Fv/Fm和Yield)、细胞密度和叶绿素相对含量显著高于20℃与30℃处理组。与20℃和25℃相比,在30℃时微绿球藻对Cd2+更加敏感,各荧光参数值均较低,细胞密度和叶绿素相对含量明显下降。杜氏盐藻在25℃时对Cd2+的耐受性最强,15℃与35℃温度条件下,各参数均明显下降,表明不适合的温度会增加重金属Cd2+的胁迫作用。本文还初步探讨了叶绿素荧光技术在重金属与环境因子联合胁迫研究中的应用。Chlorophyll fluorescence measurement has several good characteristics, such as very quick, extremely sensitive, non-invasive and non-destructive, thus becomes a widely used technique for the investigation of mechanisms of photosynthesis and to study the effects of various environmental conditions （temperature, salinity, heavy metal stress, nutrient de- ficiency） on the microalgal photosynthetic reactions, particularly for stress physiology of mi- croalgae. In this study, the interaction effects of different CD^2＋concentrations （Chlorella sp.. 0, 20, 40μmol/L;Nannochlorisoculata. 0, 10, 30μmol/L;Dunaliella salina. O, 70, 140μmol/L） and different temperature （Chlorella sp. and N. oculata： 20, 25, 30℃; D. sa- lina. 15, 25, 35℃） on the chlorophyll fluorescence parameters （Fv/Fm, Yield, qP and NPQ）, relative chlorophyll content and cell density of 3 microalgal strains were reported. The above fluorescence parameters including the ratio of variable to maximal chlorophyll flu- orescence （Fv/Fm）, which has been shown to be a sensitive indicator of photosynthetic per- formance and photoinhibition, the actual photochemical efficiency of PSII in the light （qbPSII）, the photochemical quenching （qP） and the non-photochemical quenching （NPQ）. The 3 microalgal strains used in the experiment were obtained from the Microalgae Culture Center （MACC）, Ocean University of China. Cultures were grown in sterilized seawater en- riched with f/2 medium. The salinity was adjusted to 28 by using distilled water, and the light intensity was 100μmol · m^-2 · s^-1 The results showed that the chlorophyll fluores- cence parameters, relative chlorophyll contents and cell densities all decreased obviously in the 3 microalgal strains with the increasing heavy metal （CD^2＋ ） concentrations, with the ex- ception of Fv/Fm in N. oculata, where there was no significant difference between the con- trol and the 10μmol/L Cd2＋ concentration treatment. This was primarily due to the stimula- tion alga

关 键 词：小球藻 微绿球藻 杜氏盐藻 Cd2+ 温度 叶绿素荧光 

分 类 号：Q945.78[生物学—植物学]