基于线粒体D-loop基因探讨花鳗鲡的群体遗传多样性及其种群进化历史  

Genetic variation and population evolutionary history of the giant mottled eel (Anguilla marmorata) based on the mitochondrial D-loop gene

作　　者：丁旭[1] 齐鑫[1] 尹绍武[2] 

机构地区：[1]海南大学海洋学院,海南海口570228 [2]南京师范大学生命科学学院,江苏南京210046

出　　处：《海洋科学》2012年第5期117-123,共7页Marine Sciences

基　　金："十一五"国家科技支撑计划重点资助项目(2007BAD29B03);江苏高校优势学科建设工程资助项目

摘　　要：通过测定花鳗鲡(Anguillia marmorata)海南群体(HN)和菲律宾群体(PH)共19尾个体的线粒体D-loop基因的核苷酸序列(约1017 bp),分析了花鳗鲡的种群遗传结构。结果表明:A、T、G、C 4种核苷酸的平均含量分别为39.8%、28.3%、12.4%、19.4%,A+T含量(68.1%)明显高于G+C含量(31.8%)。所测序列中存在73个变异位点,共有18个单倍型。其中海南群体的单倍型多样度(Hd)、核苷酸多态性(Pi)、平均核苷酸差异数(k)分别为0.982、0.21577和219.655,而菲律宾群体的单倍型多样度、核苷酸多态性、平均核苷酸差异数分别为1.000、0.26728和271.821,两个群体之间平均遗传距离(P)为0.3203。结果表明2个群体遗传变异较大,菲律宾群体的遗传多样性较海南群体更加丰富。通过构建NJ分子系统树表明2个群体的亲缘关系较近。利用中性检验Tajima’s D(海南群体D=1.35345,P>0.01;菲律宾群体D=0.79220,P>0.01)和Fs(海南群体Fs=3.759;菲律宾群体Fs=2.231)探讨其种群历史,表明花鳗鲡群体进化过程种群数量较为稳定。The population genetic structures of the giant mottled eel from Hainan and Philippines were investigated by sequencing the mitochondrial control region （D-loop） gene for the first time. The average contents of A, T, G and C in the control region were 39.8%, 28.3%, 12.4% and 19.4%, respectively. There were 73 polymorphisms sites from the sequenced samples revealing 18 haplotypes. By calculating the haplotype diversity （Hd）, nucleotide diver- sity （Pi） and average number ofpairwise nucleotide difference （k）, the Philippines population exhibited higher level of variability （Hd=l.000, Pi=0.26728, k=-271.821） than the Hainan population （Hd=0.982, Pi=0.21577, k=-219.655 and the genetic distance （P） was 0.3203 between two populations. By constructing the molecular phylogenetic tree with the method of N J, the two geographic populations of the giant mottled eel did not show significant genetic difference. Besides, neutrality tests indicated a possible stable population in the population history of the giant mottled eel.

关 键 词：花鳗鲡(Anguillia marmorata) 海南和菲律宾群体 线粒体D—loop基因 遗传多样性 种群进化历史 

分 类 号：S921[农业科学—渔业资源]