重组人IL-10对皮肤移植家兔血清中IL-17家族水平的影响  

Effect of recombinant human IL-10 on the level of IL-17 family cytokines in serum of rabbit model with skin graft

作　　者：戴小波[1,2] 孙万邦[2] 张磊[2] 李佳[2] 吕岩岩[2] 

机构地区：[1]广东省中医院珠海医院检验科,广东珠海519015 [2]遵义医学院珠海校区,广东珠海519015

出　　处：《临床检验杂志》2012年第5期351-354,共4页Chinese Journal of Clinical Laboratory Science

基　　金：贵州省教育厅自然科学重点培育项目(2008032)

摘　　要：目的检测皮肤移植家兔注射重组人IL-10(rhIL-10)后血清IL-17家族水平的变化,探讨IL-17家族在rhIL-10抗免疫排斥机制中的作用。方法皮肤移植家兔分6组,分别为rhIL-10低剂量组、rhIL-10高剂量组、环孢菌素A(CsA)低剂量组、CsA高剂量组、rhIL-10+CsA低剂量联合组及生理盐水组。于皮肤移植术前1 d开始用药,每只家兔连续10 d肌肉注射。各组分别于皮肤移植术前1 d、术后1、4、7、14、21、28 d取血,分离血清,以ELISA法检测血清中IL-17家族细胞因子水平。结果术后IL-17A～F水平较术前明显升高,在术后14 d达到峰值,然后逐渐降低。rhIL-10能抑制IL-17A、IL-27、IL-17F的分泌及促进IL-25的分泌,抑制强度与使用剂量成正相关。结论 IL-17家族细胞因子大量分泌,可能介导移植后的炎症反应,促进炎性因子分泌从而参与移植排斥反应。Objective To determine the changes of IL-17 family cytokines in serum of rabbit model with skin graft after injecting recombinant human IL-10（rhIL-10） and explore the effects of IL-17 family in the rhIL-10-induced mechanism of reducing immune rejection.Methods Rabbits with skin graft were divided into 6 groups,including low and high dosage of rhIL-10 groups,low and high dosage of cyclosporine A（CsA） groups,combined low dosage of rhIL-10 and CsA group and physiological saline group.Both rhIL-10 and CsA were intramusculary injected to the rabbits from the day before skin-graft for 10 consecutive days.Blood samples were collected on the day before skin-graft and the day 1,4,7,14,21 and 28 after skin-graft.The sera were separated and the levels of IL-17 family cytokines were determined by ELISA.Results The levels of IL-17A,B,C,D,E and F after skin-graft were significantly higher than those before the operation,which reached the peak values on the day 14 and then gradually decreased.rhIL-10 inhibited the secretion of IL-17A,IL-27 and IL-17F,but promote the secretion of IL-25.The inhibitory effect of rhIL-10 was positively correlated with the dosage of rhIL-10.Conclusion IL-17 family cytokines may participate in the rejective reaction of graft and mediate the inflammatory reaction after transplantation.

关 键 词：重组人白细胞介素10 皮肤移植 白细胞介素17家族 

分 类 号：R446.6[医药卫生—诊断学]