恩度联合TP方案对食管癌细胞抑制作用的实验研究  

Inhibition of endostar combined with paclitaxel and cisplatin on the proliferation of esophageal cancer cells

作　　者：赵意[1] 蔡开灿[1] 刘德纲[1] 吴华[1] 

机构地区：[1]南方医科大学南方医院胸心外科,广州市510515

出　　处：《实用医学杂志》2012年第12期1963-1965,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目资助(编号:2007B030700006)

摘　　要：目的:探讨重组人血管内皮抑素(恩度)联合紫杉醇+顺铂(TP)对食管癌细胞的抑制是否具有协同作用。方法:复苏Eca-109食管癌细胞株,传代培养,建立Eca-109食管癌细胞裸鼠移植瘤模型。3d测量1次肿瘤体积,绘制肿瘤生长曲线;测量瘤体的终末体积、重量,计算肿瘤抑瘤率;应用流式细胞仪测定肿瘤细胞凋亡率;应用免疫组化测定促凋亡蛋白Bax、抗凋亡蛋白Bcl-2的表达。结果:恩度联合TP组能明显抑制食管癌移植瘤的生长,肿瘤体积、重量小于其他组,差异有统计学意义(P<0.05);恩度联合TP组食管癌细胞凋亡率高于其他组,差异有统计学意义(P<0.05);恩度联合TP组抗凋亡蛋白Bcl-2表达低于其他组,差异有统计学意义(P<0.05);恩度联合TP组Bax表达量高于其他组,差异有统计学意义(P<0.05)。结论:恩度联合TP方案能明显抑制食管癌细胞及移植瘤的生长,优于单独应用TP组。Objective To investigate the anti-tumor effects of recombinant human endostatin （endostar） combined with paclitaxel and cisplatin （TP） on esophegeal carcinoma cells. Methods Eca-109 esophageal carcinoma cell lines were suhcuhured until cells in logarithmic phase, then the rat models with esophageal carcinoma cells Eca-109 xenografts were constructed, the anti-tumoral effects of control group （saline）, endostar group, TP group, endostar combined with TP group were observed. The tumor weigh and volume were measured, the inhibition rate of tumor growth curve was calculated, the cell apoptotic rate was detected by flow cytometry, and the expressions of apoptotic protein Bax and Bcl-2 were detected by immunohistochemistry staining. Results Endostar combined with TP can obviously inhibit the growth of transplanted tumor, and tumor size and weight were lower, and the apoptotic rate was higher than those in the other groups （P 〈 0.05）. The expression of Bcl-2 was lower, while Bax was higher in endostar combined with TP group than those in the other groups （P 〈 0.05）. Conclusion Endostar combined with TP could significantly inhibit the growth of esophageal carcinoma cells and tumor, which was better than the application of TP.

关 键 词：食管肿瘤 重组人血管内皮抑素 恩度 紫杉醇 顺铂 裸鼠 

分 类 号：R735.1[医药卫生—肿瘤]