HPLC测定氟康唑莫西沙星眼用即型凝胶的含量  

Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC

作　　者：吴明钗[1] 沈秀微[1] 林洁[1] 谢丽晓[1] 黄鹏[1] 易林高[1] 林思思[2] 

机构地区：[1]温州医学院附属第三医院,浙江瑞安325200 [2]温州医学院,浙江温州325000

出　　处：《中国现代应用药学》2012年第6期534-536,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：温州市科技计划项目(Y20110193)

摘　　要：目的建立高效液相色谱法测定氟康唑莫西沙星眼用即型凝胶含量,同时测定制剂中氟康唑、莫西沙星和羟苯乙酯的含量。方法采用ODS-C18色谱柱,以三乙胺缓冲液-甲醇为流动相,流速1.0 mL.min 1,检测波长260 nm。结果氟康唑、莫西沙星、羟苯乙酯的线性范围分别为25～250 g.mL 1(r=0.999 9,n=6),25～250 g.mL 1(r=0.999 9,n=6),2.5～25 g.mL 1(r=0.999 7,n=6),平均回收率分别为99.6%,99.4%,99.3%。RSD分别为1.23%,1.20%,1.17%。结论本方法操作简便,灵敏度高,重复性好,可用于该制剂的含量测定。OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C18 column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min^-1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25-250 μg·mL-l（r=0.999 9, n=6）, 25-250 μg·mL-l（r=0.999 9, n=6）, 2.5-25 p.g.mL-1 （r=0.999 7, n=6）, the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation.

关 键 词：高效液相色谱法 含量测定 氟康唑 莫西沙星 羟苯乙酯 

分 类 号：R965.1[医药卫生—药理学]