从干烟草叶中超声波辅助提取绿原酸的正交设计及优化  

Optimization and orthogonal design of an ultra- sonic-assisted aqueous extraction process for extracting chlorogenic acid from dry tobacco leaves

作　　者：Martin Tongai Mazvimba 于颖[1] 崔志芹[1] 张莹[1] 

机构地区：[1]中国药科大学制药装备与自动化教研室,南京210009

出　　处：《中国天然药物》2012年第4期311-320,共10页

摘　　要：本文对从烟草叶中提取绿原酸的热回流及超声波辅助提取技术进行了过程参数优化。建立了两种方法的操作条件，提取溶剂及提取率的优化过程。尽管甲醇作为溶剂进行热回流提取时效率较高，但是提取物中有溶剂成分残留。为了克服这个缺点，采用了一种新的以水为溶剂的超声波辅助提取技术，同时提出了一种小容量溶剂的提取方法，以提高干烟草叶中绿原酸的溶出率。这一超声波辅助提取过程的结果令人满意。Processing parameters for heat reflux and ultrasonic-assisted extraction techniques were optimized. Optimal operating conditions, extraction solvents and extraction yields for both methods were established. Although methanol showed high extraction efficiency in heat reflux extraction, residual amounts of methanol caused adulteration of extracts. To circumvent this drawback, a novel ultrasonic-assisted aqueous extraction process was optimized and orthogonally designed to pave the way for replacing the toxic organic solvent, methanol with water. A new approach which utilizes fractional volumes of an extraction solvent was developed to minimize solvent consumption, improve chlorogenic acid solubility in water and enhance its aqueous extraction from dried tobacco leaves. Desired trajectories for the new ultrasonic assisted aqueous extraction process were found.

关 键 词：烟草叶 绿原酸 热回流提取 超声波辅助提取 小容量 

分 类 号：R284.2[医药卫生—中药学]