猪伪狂犬病病毒SL株TK基因的克隆与生物信息学分析  

Bioinformatics Analysis of TKGene of Pseudorabies Virus SL Strain from Pigs

作　　者：张士强[1] 易悦[1] 徐志文[1] 朱玲[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014

出　　处：《中国畜牧兽医》2012年第6期26-30,共5页China Animal Husbandry & Veterinary Medicine

基　　金：科技部国家科技支撑计划(2006BAD06A18);教育部长江学者和创新团队发展计划项目(IRT0848)

摘　　要：本试验对伪狂犬病病毒SL株(四川分离株)TK基因进行克隆,并对TK基因的同源性、遗传进化树、密码子偏嗜性、氨基酸结构及亲疏水性、跨膜区、信号肽进行了分析。结果显示,本研究成功克隆了PRV SL株TK全基因,其编码区长963bp,编码320个氨基酸残基,与其他PRV分离株核苷酸序列同源性超过99.3%,编码的多肽链中疏水区域大于亲水区域,不含跨膜区及信号肽,不存在于病毒囊膜。TK gene of pseudorabies virus SL strain was cloned. Based on the sequence, the homology, phylogeny tree, co- don bias, amino acid structure, hydrophilic and hydrophobicity, transmembrane region and signal peptide were analyzed. The results showed that TK gene was successfully cloned and it consisted 963 nucleotides encoding a protein of 320 amino acids. The TK gene shared over 99.3% homology with that of the other PRV strains. PRV TK was predicted to not have the trans- membrane region and signal peptide, and not be inside of virus envelope.

关 键 词：伪狂犬病病毒 TK基因 生物信息学分析 

分 类 号：Q78[生物学—分子生物学]