犬瘟热病毒贵州株(CDV-GZ1)F基因的克隆及序列分析  

Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou

作　　者：曾智勇[1,2] 周莉[1] 刘志杰[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病研究室,贵州贵阳550025

出　　处：《中国畜牧兽医》2012年第6期53-56,共4页China Animal Husbandry & Veterinary Medicine

基　　金：贵州大学博士基金"犬瘟热病毒贵州株的分离鉴定及主要抗原蛋白编码基因的研究"(X060054)

摘　　要：根据GenBank中发表的犬瘟热病毒Onderstepoort株融合蛋白基因(F)序列设计两对特异性引物,采用RT-PCR扩增犬瘟热病毒贵州分离株(CDV-GZ1)的F基因,并进行克隆与序列分析。结果显示,CDV-GZ1株F基因的ORF全长1989bp,氨基酸序列中具有5个潜在N-糖基化位点,氨基酸同源性与美国00-2601株和国内HL株较高,分别为91.6%和91.1%,表明该分离株与00-2601株和HL株具有较近的亲缘关系。Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank, and the F gene was amplified by reverse transcription polymerase chain reaction （RT- PCR） from CDV-GZI strain. The amplified fragment was cloned and analyzed. The results showed that the length of CDV- GZ1 F gene was 1989 bp. It contained five potential N-glycosylation locations based on the derived amino acid sequences. The homology of coding amino acid between this strain and American 00-2601 and domestic HL strain was 91.6 % and 91. 1% respectively, it showed that they had common ancestor.

关 键 词：犬瘟热病毒 CDV-GZ1 F蛋白 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]