一株基因Ⅸ型鸭源新城疫病毒F基因的克隆及序列分析  

Cloning and Sequence Analysis of F Gene of a Genetype Ⅸ Newcastle Disease Virus Isolated from Duck

作　　者：袁远华[1,2] 王俊峰[1,2] 蔡丽丽[1,2] 黄兴国[2] 梁小英[2] 曹伟胜[1] 黄淑坚[2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科学技术学院生命科学学院,广东佛山528231

出　　处：《中国畜牧兽医》2012年第6期61-64,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技厅农业攻关项目(2009B020418003)

摘　　要：从广东地区疑似患有新城疫的病死雏番鸭群中分离到1株新城疫病毒,命名为GD1002株。毒力测定结果表明,致死鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)、6周龄鸡静脉接种致病指数(IVPI)分别为56.0h、1.64、2.29,符合强毒株的毒力判断标准。利用RT-PCR扩增了该分离株F基因的主要功能区片段,并进行了克隆与序列分析。该分离株F蛋白裂解位点的组成为112 R-R-Q-R-R-F117,具有典型新城疫强毒株的分子特征。F基因第47-420nt片段基因分型分析表明,该流行株属于基因Ⅸ型。相似性及遗传进化分析结果表明,该分离株与基因Ⅸ型毒株F48E9、JS/1/97/Ch和FJ/1/85/Ch株有较高同源性和较近的亲缘关系。Newcastle disease virus strain GD1002, isolated from the diseased ducks in Guangdong province in 2010, was velogenic according to the pathogenetic tests and highly pathogenicity to duck. The results of virulence determination showed that the MDT, ICPI and IVPI were 56.0 h, 1.64 and 2.29, respectively, belonged to velogenic strain virulence criterion. The F gene of GD1002 strain was amplified by reverse transcription potymerase chain reaction （RT-PCR）. The cleavage motif ^112 R- R QR R-F^117 of F protein was identical to the characteristic of velogenic strain. For genotyping, phylogenetic tree based on the nucleotide 47 to 420 nt of the F gene were performed using the isolate and other NDV sequences obtained from GenBank. The results revealed that the isolate belonged to genotype Ⅸ. And the results of nucleotide sequence homology and evolutionary processes analyses showed that the isolate had a close relationship with F48 E9, JS/1/97/Ch and FJ/1/85/Ch strains.

关 键 词：鸭 新城疫病毒 F基因 克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]