RT-PCR方法检测犬瘟热的流行情况  

Epidemic of Canine Distemper Virus was Detected by RT-PCR

作　　者：裘云云[1] 施朋飞[1] 夏飞琴[1] 张保新[1] 赵凡凡[1] 王晓杜[1] 

机构地区：[1]浙江农林大学动物科技学院,杭州临安311300

出　　处：《中国畜牧兽医》2012年第6期76-78,共3页China Animal Husbandry & Veterinary Medicine

基　　金：浙江省自然科学基金(Y3110124);浙江农林大学校发展基金(2010FR080)

摘　　要：犬瘟热是由犬瘟热病毒引起的犬科烈性传染病,给患病动物带来重大危害。本研究基于犬瘟热病毒的NP蛋白基因设计特异性引物,利用RT-PCR获得287bp目的片段,经TA克隆并测序。结果表明,该片段与NCBI上公布的犬瘟热病毒NP序列(登录号:EU716322)同源性达到98%。利用该特异性的RT-PCR方法,检测杭州市及周边部分地区犬瘟热的流行情况,结果表明,整个杭州及周边地区犬瘟热病毒总阳性检出率为6.7%,杭州城区阳性个体数最多,阳性率最高达到18.8%,其他地方(临安、台州、舟山)阳性率较低。该研究为犬瘟热的综合防制提供基础数据。Canine distemper caused by canine distemper virus （CDV） is acute infection disease of canine, which caused se- vere losses. In this study, specific primers recognized CDV nucleoprotein were designed, the 287 bp production was amplified by RT-PCR. This production was cloned by TA cloning and sequenced, the results showed that there was a high homogeneity of 98% in nucleotide with canine distemper virus NP sequence （accession number： EU716322） published in the GenBank. Epi- demic of canine distemper in Hangzhou and other cities nearby was investigated by this specific RT-PCR. The results showed that total positive detection rate of CDV was 6.7%, the number of positive individuals in Hangzhou city was largest and posi- tive detection rate was most up to 18.8%, positive detection of other cities （Linan, Taizhou, Zhoushan） were lower. The study is basic to prevention and treatment of canine distemper.

关 键 词：犬瘟热 RT-PCR 杭州 流行 

分 类 号：S851.3[农业科学—预防兽医学]