一株鸡源性肠球菌的鉴定及耐药机制分析  

Isolation, Identification and Drug Resistance Analysis of a Pathogenic Enterococcus Strain Isolated from Chicken

作　　者：王涛[1] 杜冬冬[1] 庄燕飞[1] 常维山[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国畜牧兽医》2012年第6期165-168,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验旨在鉴定一株从肉鸡体内分离的广谱耐药菌,并从分子生物学角度对该株细菌的耐药机理进行探讨。根据《常见细菌系统鉴定手册》标准对该菌进行培养特性、生化试验鉴定,设计1对16SrDNA通用引物扩增其16SrDNA并测序鉴定;根据该菌的药物敏感性试验结果设计了12对引物扩增其基因组上耐药基因。细菌培养特性和生化试验结果显示该菌为一株肠球菌,PCR扩增出大小为1465bp的目的片段,药敏试验显示该株细菌耐受多种抗生素,PCR扩增其耐药基因发现存在ant(6)-iv、OXA-10、tetM、CTX-M-1、TEM基因。最终鉴定该株细菌为一株肠球菌,耐药基因的存在是其产生耐药性的原因之一。This experiment was aimed at identifying a Broad-spectrum antibiotic resistant bacteria separated from chickens and discussing the mechanism of antibiotic resistance of the bacteria from the viewpoint of molecular biology. Identify the train- ing characteristics and biochemical experiments of the bacteria based on the standard of Common Bacteria System Identification Manual. A pair of 16S rDNA general primer was designed to amplify 16S rDNA and sequenced. 12 pairs of primer amplify re- sistance gene was designed based on the results of sensitive method of antibiotic test. The results of training characteristics and biochemical experiments of the bacteria showed that they were Enterococcus. A target set of 1465 bp was amplified by PCR. Sensitivity tests showed that the bacteria tolerate several antibiotics. The results of PCR showed that there exist ant（6）-iv, OXA-10, tetM, CTX-M-1, TEM gene, so it was Enterococcus. The existence of resistance gene was one of the reasons why the bacteria generate resistance characteristics.

关 键 词：肠球菌 鉴定 耐药性 耐药基因 

分 类 号：Q852.611[生物学]