一株鸡传染性法氏囊病病毒的分离鉴定  

Isolation and Identification of One Strain Infectious Bursal Disease Virus

作　　者：赵云英[1] 张燕欣[1] 张文生[2] 李富桂[1] 

机构地区：[1]天津农学院动物科学系,天津300384 [2]天津职业大学生物与环境工程学院,天津300402

出　　处：《中国畜牧兽医》2012年第6期207-211,共5页China Animal Husbandry & Veterinary Medicine

摘　　要：从天津地区免疫失败的鸡群中分离一株IBDV(命名TJ-hg),应用血清学AGP试验结果可见明显的白色沉淀线;应用逆转录酶—聚合酶链式反应(RT-PCR)扩增的IBDVVP2基因高变区(vVP2)及其序列分析的结果表明,TJ-hg株与超强毒株的核苷酸同源性均在90%以上,氨基酸同源性达98.8%～99.3%,且高变区中特征性氨基酸的变化与超强毒株完全一致,TJ-hg株在第一亲水区氨基酸Q219P发生突变。研究结果表明,TJ-hg株属于超强毒株,但又与标准毒株存在差异。One isolate of infectious bursal disease virus （IBDV） ,was isolated from the vaccination failure chicken. Through serologic AGP experiment,results showed obvious white precipitation line; the high variable （hv） region of VP2 genes were amplified by RT-PCR （reverse transcriptase polymerase chain reaction） and were compared for their sequences. It was indica- ted that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90% and 98.8% to 99.30/oo at nucleotide level,respectively. And the change of amino acid was complement in high variable （hv） region of VP2 genes. The mutations a- mino acid Q219P located in the first hydrophilic region of TJ-hg strain. The research demonstrated that TJ-hg strain belonged to vvIBDV,but it was different from standard strain.

关 键 词：IBDV 超强毒株 分子鉴定 

分 类 号：S852.65[农业科学—基础兽医学]