郏县红牛子宫内细菌的分离与鉴定  

Isolation and Identification of Bacteria from Uterus of Jiaxian Red Cattle

作　　者：秦佳晨[1] 曹桂彬[1] 石凯文[1] 邓立新[1] 石奎林 李志刚[1,2] 秦瑞峰[1,2] 王新庄[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]平顶山郏县红牛繁育中心,河南平顶山467000

出　　处：《西北农业学报》2012年第4期21-25,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：现代农业(肉牛)产业技术体系建设专项基金(0300324);河南省标准化创建补贴基金

摘　　要：研究郏县红牛产后子宫内细菌的分部情况,为牛子宫内膜炎的预防、治疗提供依据。通过对产后不同时间的21份子宫内容物进行分离、纯化、鉴定,结果共分离出细菌14种(43株),其中以蜡样芽孢杆菌、大肠埃希菌、产吲哚金黄杆菌、浅黄金色单胞菌、奥斯陆莫拉菌、葡萄球菌等为主。从革兰氏染色结果来看,革兰氏阳性菌有23株(占53.5%)、革兰氏阴性菌有20株(占46.5%)。结果与奶牛子宫内膜炎患病牛所分离的细菌菌株及革兰氏染色特性相一致。In order to provide the basis for prevention and treatment of cattle endometritis, bacteria di- vision in postpartum uterus of Jiaxian red cattle was analyzed. As the result 14 bacteria species （43 strains） were isolated from 21 postpartum uterus contents after the separation, purification and identi- fication process. The main species are Bacillus cereus, Escherichia coli, Chryseobacterium indolo- genes, Chryseornonas luteola, Moraxella osloensis, Staphylococcus. After Gram staining test, 23 strains （53.5%） were Gram-positive bacteria, and 20 strains （46.5%） were Gram-negative bacteria. The bacteria strains and Gram staining results are consistent with previous results, in which bacteria were isolated from cows with endometritis.

关 键 词：郏县红牛 细菌分离 鉴定 

分 类 号：S823[农业科学—畜牧学]