哈密地区瓜列当茎腐病病原菌的分离与鉴定  

Isolation and Identification of Pathogen of Orobanche aegyptiaca Stem-rot Disease in Hami

作　　者：丁丽丽[1] 张静[2] 王兴应[3] 张学坤[1] 李全喜[3] 赵思峰[1] 

机构地区：[1]石河子大学,绿洲农作物病害防控重点实验室,新疆石河子832003 [2]新疆生产建设兵团农十三师农业科学研究所,新疆哈密839000 [3]新疆生产建设兵团农十三师淖毛湖农场,新疆伊吾839000

出　　处：《西北农业学报》2012年第4期187-192,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：新疆生产建设兵团科技攻关计划(2008GG03);新疆生产建设兵团博士基金(2011BB01);石河子大学自然科学与技术创新项目(ZRKX2009YB07)

摘　　要：为了查清新疆哈密地区瓜列当茎基腐病的主要种类,从哈密地区采集寄生在甜瓜上的瓜列当茎基腐病样品,对病原物进行分离、纯化和致病性测定,并对病原物进行形态学和分子生物学鉴定。结果表明,从130个样品上共计分离获得105个分离物,其中腐霉菌39个,占分离总数的37.14%;丝核菌34个,占32.38%;镰刀菌21个,占20%;其他11个,占10.48%。依据形态学特征和ITS序列分析鉴定腐霉菌,得到2个种,分别为瓜果腐霉(Pythium aphanidermatum)和终极腐霉(Pythium ultimum)。瓜果腐霉是引起哈密地区瓜列当茎基腐病的主要致病菌。To identify the pathogen of Orobanche aegyptiaca stem-rot disease in Hami in Xinjiang province,the stem-rot samples of Orobanche aegyptiaca which parasite on melon were collected from Hami regions.The pathogens were isolated and the pathogenicity was determined.One hundred and five isolates were obtained from 130 samples of stem-rot diseased O.aegyptiaca plant.According to morphological features of culture,39 of them,37.14% of the total isolates,belong to Pythium;34 of them,32.38% of the total isolates,belong to Rhizotonia and 21 of them,20% of the total isolates,belong to Fusarium;and 11 of them,10.48% of the total isolates,belong to others.The Pythium isolates had high virulence to O.aegyptiaca plant which was the main pathogen for stem-rot of O.aegyptiaca in Xinjiang.These isolates were classified into two different species,e.g.Pythium aphanidermatum（Edson） Fitzp,and Pythium ultimum according to the morphological features of culture and the ITS sequence analysis of rDNA of representative strains.The P.aphanidermatum is key pathogen of Orobanche aegyptiaca stem-rot disease in Hami.

关 键 词：瓜列当 茎基腐病 腐霉菌 rDNA-ITS区序列分析 鉴定 

分 类 号：S476.7[农业科学—农业昆虫与害虫防治]