异丙酚后处理对大鼠脑缺血再灌注损伤的影响：长期观察  

Effect of propofol postcondifioning on cerebral ischemia-reperfusion injury in rats： a long-term observation

作　　者：文志廷[1] 王国林[1] 王海云[1] 李翠[1] 罗猛强[1] 

机构地区：[1]天津医科大学总医院麻醉科,300052

出　　处：《中华麻醉学杂志》2012年第4期411-415,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81071059）

摘　　要：目的评价异丙酚后处理对大鼠脑缺血再灌注损伤的影响：长期观察。方法健康雄性sD大鼠144只，体重250～280g，7—8周龄，采用随机数字表法，将其随机分为4组（n=36）：假手术组（S组）、缺血再灌注组（I／R组）、异丙酚后处理组（P组）和溶媒对照组（I组）。I／R组、P组和I组采用线栓法阻塞大脑中动脉60min进行再灌注的方法制备局灶性脑缺血再灌注损伤模型。P组在再灌注即刻开始静脉输注异丙酚20Ⅱ1g·kg^-1·h^-1 2h，S组和I／R组给予等容量生理盐水，I组给予等容量10％脂肪乳。4组分别于术后1、14、28d取5只大鼠，进行神经功能评分及脑梗死体积测定；4组各取6只大鼠，分别于术后9、23d开始进行Morris水迷宫测试，连续6d；4组分别于术后1、14、28d取5只大鼠，取海马组织，测定使君子酸（AMPA）受体GIuR1亚基及其在胞膜上表达水平，计算二者比值（胞膜GIuR1亚基／GIuR1亚基）。结果与s组比较，I／R组神经功能评分和跨越平台次数降低，脑梗死体积增加，逃避潜伏期延长，胞膜GIuR1亚基／GIuR1亚基增加（P〈0．05），而海马组织GIuR1亚基表达差异无统计学意义（P〉0．05）；异丙酚后处理可抑制脑缺血再灌注诱导的上述改变（P〈0．05）。结论异丙酚后处理减轻大鼠脑缺血再灌注损伤的效应可连续到术后28d，其部分机制与抑制含GIuR1亚基AMPA受体向细胞膜转运有关。Objective To investigate the long-term effects of propofol postconditioning on cerebral ischemia-reperfusion （I/R） injury in rats. Methods One hundred and forty-four healthy male SD rats, aged 7-8 weeks, weighing 250-280 g, were equally and randomly divided into 4 groups： sham operation group （group S）, I/R group, propofol posteonditioning group （group P） and intralipid group （group I）. The animals were anesthe- tized with intraperitoneal 10% chloral hydrate 300 mg/kg. Focal cerebral ischemia was induced by occlusion of middle cerebral artery for 60 min using a nylon thread with a rounded tip which was inserted into internal carotid artery in groups I/R, P and I. Two hour infusion of propofol was started at 20 mg＇kg-1 ＂h-l immediately after the onset of reperfusion in group P, while the equal volume of normal saline was given instead in S and I/R groups, and 10% intralipid was given instead in group I. Five rats in each group were chosen on day 1, 14 and 28 after operation for assessment of neurological behavior and detection of cerebral infarct volume, six rats in each group were chosen to perform Morris water maze test at day 9 and 23 after operation for 6 consecutive days. Five rats in each group were sac.~ced on day 1, 14 and 28 after operation and the hippocampal tissues were isolated for deter- mination of the expression of GluRl-containing AMPA （GluR1-AMPA） receptor and GluR1-AMPA receptor in cell membrane. The ratio of GluR1-AMPA receptor in cell membrane/GluR1-AMPA receptor was calculated. Results Compared with group S, neurological behavior scores and the number of animals＇ swimming across the platform were significantly decreased, cerebral infarct volume was significantly enlarged, escape latency was significantly prolonged, and ratio of GluR1-AMPA receptor in cell membrane/GluR1-AMPA receptor was significantly increased （ P 〈 0.05）, while no significant change in the expression of GluR1-AMPA receptor was found in I/R group （ P 〉 0.05）. Propofol postconditioning

关 键 词：二异丙酚 再灌注损伤 脑 受体 AMPA 

分 类 号：R965[医药卫生—药理学]