新生大鼠氯胺酮麻醉对成年后认知功能的影响  

Neonatal ketamine exposure-induced cognitive dysfunction persists into adulthood in rats

作　　者：郭东勇[1] 谭涛[2] 田心[2] 王国林[3] 

机构地区：[1]天津医科大学附属肿瘤医院麻醉科天津市肿瘤防治重点实验室,300060 [2]天津医科大学生物医学工程学院基础医学研究中心神经工程实验室,300060 [3]天津医科大学总医院麻醉科,300060

出　　处：《中华麻醉学杂志》2012年第4期420-422,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价新生大鼠氯胺酮麻醉对成年后认知功能的影响。方法SD大鼠30只，雌雄各半，7d龄，采用随机数字表法，将大鼠随机分为3组（n=10）：对照组（C组）、氯胺酮浅麻醉组（K1组）和氯胺酮深麻醉组（K2组）。K1组和K2组分别腹腔注射氯胺酮25和50mg／kg（生理盐水稀释至0．2m1），C组腹腔注射等容量生理盐水。于2．5月龄时，行水迷宫实验，记录逃避潜伏期，计算在平台所在象限游泳时间占整个游泳时间的百分比（L／T总），1周后，行长时程增强（LTP）测试。结果3组逃避潜伏期和T3/T总比较差异无统计学意义（P〉0．05）。与C组比较，K1组和K2组LTP水平降低（P〈0．05），K1组和K2组LTP水平差异无统计学意义（P〉0．05）。结论新生大鼠氯胺酮麻醉可降低其成年后认知功能，且不同麻醉深度降低认知功能程度无差异。Objective To determine if cognitive dysfunction induced by neonatal ketamine anesthesia can persist into adulthood. Methods Thirty 7-day-old SD rats of both sexes were randomly divided into 3 groups. Control group received normal saline 0.2 ml intraperitoneally （IP） （group C）, while ketamine groups 1 and 2 received ketamine 25 and 50 mg/kg ]P respectively （groups K1 , K2 ）. Morris water maze test was performed and hippocampal long-term potentiation （LTP） recorded when the rats were 2.5 months old. Results LTP was significantly lower in groups K1 and K2 than in group C. There was no significant difference in LTP between groups K1 and K2. The escape latency and swimming time were comparable among the 3 groups. Conclusion Neonatal ketamine anesthesia can decrease the cognitive function in adult rats. There is no significant difference between light and deep ketamine anesthesia.

关 键 词：氯胺酮 认知障碍 婴儿 新生 成年人 

分 类 号：R726.1[医药卫生—儿科]