正交试验优选香草固齿酊的提取工艺  

Optimization of Extraction Technology for Xiangcao Guchi Tincture by Orthogonal Test

作　　者：王岩[1] 吴淑媛[1] 廖祈祈[1] 林丽珍[1] 郭小媚[1] 谢谦[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《中国实验方剂学杂志》2012年第12期56-59,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省中医药局科研基金(2010202)

摘　　要：目的:优选香草固齿酊的提取工艺。方法:采用乙醇渗漉法,以提取液中α-香附酮、蟛蜞菊内酯的含量及干膏得率为指标,采用HPLC测定含量,烘干法测定浸膏得率,正交试验优选提取工艺条件,对乙醇体积分数、浸渍时间等因素进行考察。结果:最佳提取工艺为60%乙醇浸渍渗漉24 h,流速5 mL.min-1。结论:该优选工艺稳定可行,适用于工业化生产。Objective： To optimize extraction technology of Xiangcao Guchi tincture. Method： Using the content of α-cyperone and wedelolactone in extraction liquid and yield of dry extract as indexes, the content of them were determined by HPLC, yield of extract was determined by baking method, ethanol percolation method was used, orthogonal test was used to optimize extraction process conditions with the concentration of ethanol, immersion time and velocity as factors. Result： Optimum extraction conditions were as follows： The concentration of ethanol 60% , velocity was 5 mL·min-l, and impregnated percolation time was 24 h. Conclusion： Optimized extraction technology was stable and reasonable, it could apply to industrial production.

关 键 词：香草固齿酊 Α-香附酮 蟛蜞菊内酯 正交试验 

分 类 号：R283.6[医药卫生—中药学]