紫外分光光度法测定青天葵药材总黄酮含量  

Total Flavonoids Determination of Nervilia fordii by Ultraviolet Spectrophotometry

作　　者：徐灵源[1] 谢集照[1] 谢云峰[1] 邱莉[1] 秦箐[1] 黄桂坤[1] 谢婷[1] 

机构地区：[1]广西医科大学药学院,南宁530021

出　　处：《中国实验方剂学杂志》2012年第12期88-90,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学青年基金项目(桂科0991026);广西医科大学博士科研启动基金项目(304203)

摘　　要：目的:建立一种青天葵药材中总黄酮含量测定的方法,并考察14批青天葵药材中总黄酮的含量。方法:以芦丁为对照品,AlCl3-HAc-NaAc(pH 5.5)为显色体系,采用紫外分光光度法于最大吸收波长405 nm处测定。结果:芦丁在7.28～30.80 mg.L-1线性关系良好(r=1.000),总黄酮平均回收率为100.73%,RSD 1.92%。14批青天葵药材中,总黄酮含量范围为1.25%～3.12%。结论:该方法操作简便、快速、准确、稳定可靠,可用于青天葵药材中总黄酮的含量测定。Objective： A method for the quality control of Nervilia fordii was developed, and the quality of 14 batches of N. fordii purchased from different markets was investigated. Method： Rutin and A1C13-HAc-NaAc （pH 5.5 ） system were employed as reference substance and chromogenic reagent, respectively, the total flavonoids of N. fordii were measured by UV spectrophotometry at the 405 nm. Result： The linear relationship of rutin was in the range of 0. 007 28-0. 030 8 g · L-1 （r = 1. 000）. The average recovery was 99.78% （RSD 1.94% ）. The range of the amount of the total flavonoids of 14 batches of N. fordii was 1.25%-3.12%. Conclusion： This method was proved to be simple, quick, accurated, stable and reliable, and it could be used to determine the total flavonoids of N. fordii.

关 键 词：青天葵 总黄酮 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]