藏药三果汤散抗氧化有效成分的薄层色谱-生物自显影及HPLC指纹图谱  

Preliminary Study for Antioxidant Components of Tibetan Medicine Sanguo Tang San by TLC-bioautograhpy and HPLC Fingerprinting

作　　者：德洛[1] 姚喆[1] 冀静[1] 杨继家[1] 罗尚华[1] 兰莎[1] 唐策[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137

出　　处：《中国实验方剂学杂志》2012年第12期98-102,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973957;30960507);教育部博士点专项科研基金项目(20095132110008)

摘　　要：目的:初步建立藏药三果汤散的HPLC指纹图谱,研究藏药三果汤散的抗氧化活性,初步筛选出三果汤散中抗氧化有效成分。方法:采用薄层色谱-生物自显影技术和1,1-二苯基-2-三硝基苯肼(DPPH)分光光度法对不同来源的三果汤散、单味药对照药材及其中单体成分没食子酸、没食子酸乙酯、鞣花酸、柯里拉京进行抗氧化作用初步测定,并采用高效液相色谱法初步建立了三果汤散的指纹图谱。结果:不同来源的三果汤散的抗氧化作用差异不大,所含化学成分以没食子酸抗氧化作用最强,揭示没食子酸可能是三果汤散抗氧化的主要有效成分之一。结论:该方法准确、简便可行,为进一步探索三果汤散抗氧化有效成分提供了科学依据。Objective： To evaluate the antioxidant ability and preliminarily screen antioxidant components in Tibetan medicine Sanguo Tang San with TLC-bioautograhpy, HPLC fingerprinting and spectrophotography. Method： The antioxidant ability of Sanguo Tang San from different areas, single herbs and formula＇s chemical components, including gallic acid, ethyl gallate, ellagic acid and corilagin, were initially evaluated. And then the analysis methods for antioxidant components were established. Result： Sanguo Tang San from different sources had similar antioxidant activity revealed, as well as gallic acid had the best antioxidant activity of all components. It indicated that gallic acid might be one of the major antioxidant active components in Sanguo Tang San from different resources. Conclusion： This method was accurate and reliabile, which supplied scientific basis for further exploring antioxidant components of Sanguo Tang San.

关 键 词：藏药 三果汤散 抗氧化 薄层色谱-生物自显影 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]