HPLC测定不同产地单芽狗脊中山柰酚-3,7-O-α-L-二鼠李糖苷的含量  

Determination of Kaempferoα 3,7-O-α-L-dirhamnopyranoside in Woodwardiae unigemmata from Different Habitats by HPLC

作　　者：马秉智[1] 鞠海[1] 田雪峰[1] 王巍[1] 朱立平[1] 高增平[2] 

机构地区：[1]中日友好医院药学部,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2012年第12期130-131,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立高效液相色谱法测定单芽狗脊中山柰酚3,7-O-α-L-二鼠李糖苷含量的方法。方法:HPLC条件为DiamonsilTMC18(4.6 mm×200 mm,5μm)色谱柱,流动相乙腈-水(30∶70),检测波长265 nm,流速1.0 mL.min-1,柱温25℃。结果:9个产地单芽狗脊中山柰酚3,7-O-α-L-二鼠李糖苷的质量分数范围为0.05%～0.43%。结论:该法简便、准确、专属性强,可用于单芽狗脊中山奈酚-3,7-O-α-L-二鼠李糖苷的含量测定。Objective： To develop a HPLC method for the content determination of kaempferol 3,7-O-α-L-dirhamnopyranoside in Woodwardiae unigemmata. Method： HPLC was applied with a DiamonsilTMCls column by aeetonitrile-water （30： 70） as mobile phase. The flow rate Was 1.0 mL · min-1. The column temperature was at 25 ℃ and the UV detection wavelength was at 265 nm. Result： The content range of kaempferol 3,7-O-α-L- dirhamnopyranoside from 9 different habats was from 0.05 % -0.43 %. Conclusion ： The method is simple, accurate and specific. It can be used for determination of kaempferol 3,7-O-α-L-dirhamnopyranoside in IV. unigemmata.

关 键 词：单芽狗脊 山柰酚-3 7-O-α-L-二鼠李糖苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]