鞣花酸片在家兔体内药代动力学研究  

Pharmacokinetics of Ellagic Acid Tablets in Rabbits

作　　者：裴贵珍[1] 陈文[1] 张华[1] 李桂华[1] 

机构地区：[1]新疆石河子大学药学院教育部重点实验室,新疆石河子832002

出　　处：《中国实验方剂学杂志》2012年第12期136-138,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：兵团医药专项课题(2011BA056)

摘　　要：目的:建立鞣花酸片血药浓度的高效液相色谱测定方法,并进行该药物在家兔体内的药代动力学研究。方法:采用Agilent C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.1%磷酸水(23∶77)为流动相,流速0.8 mL.min-1,检测波长254nm,柱温30℃。结果:家兔血浆中,鞣花酸的线性范围为0.013～1.635 mg.L-1(r=0.998 9),方法回收率>95%,最低检测限为0.010 mg.L-1,家兔口服给药后,鞣花酸的药时曲线出现双峰,在家兔体内存在肝肠循环。结论:实验建立了家兔血浆中鞣花酸含量的HPLC测定方法,专属性强,可用于该药在家兔体内的药动学研究。Objective ： To develop an HPLC method for the determination of ellagic acid tablets in rabbits plasma and to study the pharmacokinetics of ellagic acid tablets in rabbits. Method： An Agilent C18 （4.6 mm × 250 mm, 5 μm） column was adopted, the mobile phase was a mixture of acetonitrile and 0. 1% phosphoric acid solution （23： 77） at the flow rate of 0. 8 mL. min -1, the detection wavelength was set at 254 nm and the column temperature was controlled at 30 ℃. Result： The retention time of ellagic acid was observed at 8.5 min. The linear range of calibration curve was within drug concentrations of 0. 013-1. 635 mg · L^-1 （ r = 0. 998 9）. The quantitation limit was 0. 010 mg· L^-1. The recoveries of three levels were more than 95%. The plasma ellagic acid concentration-time curve could be evaluated by the two compartment model. Conclusion： The developed HPLC method for the pharmacokinetic study of ellagic acid tablets is precise.

关 键 词：鞣花酸片 药代动力学 高效液相色谱 

分 类 号：R285.5[医药卫生—中药学]