抵挡汤对糖尿病肾病大鼠肾脏ICAM-1作用的影响  

Effects of Didang Decoction on Expression of Intercellular Adhesion Molecular-1 in Diabetic Nephropathy Rats

作　　者：高若愚[1] 曲竹秋[2] 常柏[3] 

机构地区：[1]天津医科大学,天津300070 [2]天津医科大学总医院中医科,天津300052 [3]天津医科大学代谢病医院,天津300070

出　　处：《中国实验方剂学杂志》2012年第12期143-145,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973753)

摘　　要：目的:探讨抵挡汤对糖尿病大鼠肾脏细胞间黏附分子-1(ICAM-1)的影响。方法:利用链脲佐菌素(STZ)尾静脉注射,配合高脂饲料饮食制备糖尿病大鼠模型,将大鼠分为正常对照组、模型对照组、抵挡汤组、罗格列酮组,连续灌胃给药12周,模型组、正常组给子等量生理盐水。治疗第12周末测定各组大鼠血糖、血肌酐水平及24尿蛋白,免疫组化染色观察各组大鼠肾脏细胞间黏附分子-1(ICAM-1)的阳性表达。结果:与正常对照组比较,模型对照组大鼠肾脏ICAM-1表达增多;而与模型对照组比较,抵挡汤组和罗格列酮组的大鼠肾脏免疫组化染色ICAM-1表达减少。结论:抵挡汤抑制糖尿病大鼠肾脏ICAM-1表达,可能是其防治糖尿病肾病发生发展的机制之一。Objective： To investigate influence of Didang decoction on the low extremity vascular disease of STZ-indueed diabetic rats. Method： After being injected streptozotoein （STZ） into the caudal vein and fed with the high fat diet, diabetes model rats were divided into normal control group, model control group, Didang decoction group, and the rosiglitazone group. After continuous stomach perfusion administration for 12 weeks, the change of blood glucose, total urinary protein in 24 h, serum creatinine were detected with correlative methods, and the expression of intercellular adhesion molecule-1 （ ICAM-1 ） in kidney were examined by inmunohistoehemieal technique. Result： Compared with normal control group, the expression of ICAM-1 in model control group is significantly increased. Compared with model control group, the expression of ICAM-1 is decreased in Didang decoction group, and rosiglitazone group. Conclusion： Didang decoction can restrain expressions of ICAM-1 on kidney, maybe one of the possible mechanisms.

关 键 词：糖尿病肾病 抵挡汤 细胞间黏附分子-1 

分 类 号：R285[医药卫生—中药学]