重用赤芍治疗ANIT诱导大鼠急性淤胆型肝炎的研究  

Investigation of High Does of Paeoniae Radix Rubra Against ANIT-induced Acute Cholestasis Hepatitis in Rats

作　　者：魏思思[1,2] 赵艳玲[1] 江凤娟[1,2] 邢小燕[1,2] 朱云[1] 贾雷[1,2] 程丹红[1,2] 李瑞生[3] 肖小河[1] 

机构地区：[1]解放军第三0二医院全军中药研究所,北京100039 [2]成都中医药大学,成都610075 [3]解放军第三O二医院实验动物中心,北京100039

出　　处：《中国实验方剂学杂志》2012年第12期151-155,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173571)

摘　　要：目的:观察不同剂量赤芍对α-萘异硫氰酸酯(α-naphthylisothiocy,ANIT)诱导大鼠急性淤胆型肝炎的保护作用。方法:赤芍高、中、低(分别相当于原生药量36,18,1 g.kg-1)剂量ig给药3 d(2次/d)后,采用ANIT(60 mg.kg-1)诱导大鼠急性淤胆型肝炎模型,造模后继续给药3次,末次给药1 h后,进行胆管引流,记录大鼠6 h内的胆汁流量,检测血清学指标变化及肝脏病理学改变,结合对应分析综合评价赤芍对ANIT诱导大鼠急性淤胆型肝炎的影响。结果:血清生化结果显示,与模型组相比,赤芍中、高剂量给药组血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、碱性磷酸酶(ALP)、总胆汁酸(TBA)明显降低(P<0.01),胆汁流量显著升高(P<0.05),低剂量组变化不明显;病理组织学观察结果显示,与模型组相比,赤芍中、高剂量组肝脏病变均有明显改善,低剂量组改善不明显;对应分析结果表明,赤芍中、高剂量组的治疗效果显著,且呈剂量依赖性增强。结论:高、中剂量赤芍(36,18 g.kg-1)对ANIT诱导的大鼠急性淤胆型肝炎有显著的保护作用。Objective： To observe the protective effect of Paeoniae Radix Rubra against ANIT-induced acute cholestasis hepatitis in rats. Method： The rat cholestasis hepatitis model was induced by ANIT （60 mg· kg-1） once after intragastric administration （ig） for 3 days with Paeoniae Radix Rubra group. The extract of Paeoniae Radix Rubra was administered intragastricly after ANIT treated for three times. Blood sample was obtained, the serum biochemistry indicators were tested, and bile flow within 6 h in rats and histopathological changes in liver were also investigated. The protective effect of Paeoniae Radix Rubra was evaluated by correspondence analysis. Result： Biochemical results showed obviously that, compared with model group, the concentrations of serum alanine aminotransferase （ ALT）, aspartate aminotransferase （ AST）, total bilirubin （TBIL）, direct bilirubin （DBIL）, alkaline phosphatase （ALP）, total bile acid （TBA） in high-and mediumdose group of Paeoniae Radix Rubra were significantly decreased （P 〈 0.01 ） , and the bile flow volume was significantly increased （P 〈 0.05）. By examining the liver histopathology, it was found that hepatic cellular change, necrosis and inflammatory cell infiltration were notably alleviated in the high-and medium-dose group of Paeoniae Radix Rubra with ANIT treatment, however, the improvement was not obviously found in the low-dose group. Correspondence analysis results showed that the treatment effect was remarkable in the high-and medium- dose. Conclusion： It showed that the high-and medium-dose of Paeoniae Radix Rubra （36, 18 g· kg-1 ） have obvious treatment effect against acute cholestasis hepatitis induced by ANIT.

关 键 词：赤芍 保护作用 Α-萘异硫氰酸酯 急性淤胆型肝炎 对应分析 

分 类 号：R285.5[医药卫生—中药学]