扶正解毒颗粒对染镍大鼠尿微量蛋白及尿酶活性的影响  

Effect of Fuzheng Jiedu Granula on Urinary Micro-protein and Urinary Enzyme in Rats Exposed to Nickel

作　　者：滕玉莲[1] 吕晓云[2] 赵健雄[2] 

机构地区：[1]兰州大学校医院,兰州730000 [2]兰州大学中西医结合研究所,兰州730000

出　　处：《中国实验方剂学杂志》2012年第12期170-172,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：甘肃省自然科学研究基金计划项目(096RJZA060);兰州大学医学科研基金项目(LZUYX200805)

摘　　要：目的:观察扶正解毒颗粒(FJG)对硫酸镍(NiSO4)暴露肾损伤大鼠的保护作用。方法:50只Wistar大鼠采用NiSO42.5 mg.kg-1.d-1ip(连续7 d,此后间日1次)制备肾损伤模型,随机分为NiSO4组(模型组)、FJG高、中、低剂量组(20,10,5 g.kg-1.d-1)、二巯基丁二酸(DMSA,50 mg.kg-1.d-1)组。另设NS组(空白对照)及FJG对照组(FJG 10 g.kg-1.d-1)。各组大鼠ig 1次/d,共4周。分别采用放射免疫法(RIA)、光电比色法及酶法检测尿α1-微球蛋白(α1-MG)、N-乙酰-β-D氨基葡萄糖苷酶(NAG)及尿肌酐(UCr)的变化。结果:NiSO4组尿α1-MG及NAG活性(NAG/UCr)较NS组显著增高(P<0.05和P<0.01),与NiSO4组相比,FJG各组尿α1-MG及NAG活性则明显降低,尤以FJG高、中剂量组为著(P<0.05和P<0.01)。结论:尿α1-MG及NAG活性增高与早期镍性肾损伤存在关联,中药FJG对镍性肾损伤大鼠具有较好的保护作用。Objective： To study the effect of Fuzheng Jiedu granula （FJG） on the renal injury in rats exposed to nickel sulfate （NiSQ）. Method： Fifty Wistar rats were made into nephrotoxicity models by intraperitoneal injection of NiSO4 （2.5 mg ·kg^-1·d^-1） . They were randomized into 5 groups, model group, high dose of FJG group （20 g·kg^-1·d^-1）, middle dose of FJG group （10 g·kg^-1·d^-1）, low dose of FJG group （5 g·kg^-1·d^-1）, meso-2, 3-dimercaptosuccinic acid （DMSA） group （0.05 g·kg^-1·d^-1）. Twenty Wistar rats were divided into NS group and FJG control group （10 g·kg^-1·d^-1 ） randomly. The rats in each group were treated with intragastric administration once every day for four weeks. Urinary α1-microglobulin （α1-MG） , urinary N-acetyl-β-D-glucosaminidase （NAG） and urinary creatinine were detected by radioimmunity assay （RIA） , photoelectric colorimetry assay and enzymic assay, respectively. Result： In NiSO4 group, urinary α1-MG and NAG activity （NAG/ UCr） were increased remarkably （P 〈0.05, P 〈0.01）. Compared with that of NiSO4 group, the indexes montioned above in FJG groups decreased obviously （P 〈 0. 05, P 〈 0. 01 ）. Conclusion： Urinary α1-MG and NAG activity enhancement could be correlated with nickel-induced nephrotoxicity. FJG could protect rats from the renal injury induced by nickel.

关 键 词：扶正解毒颗粒 硫酸镍 Α1-微球蛋白 N-乙酰-Β-D氨基葡萄糖苷酶 

分 类 号：R285.5[医药卫生—中药学]