艾叶不同提取物对小鼠原发性痛经的影响  

Influence of the Different Extracts of Artemisiae argyi on Primary Dysmenorrheal Modelin Mice

作　　者：张来宾[1] 阎玺庆[1] 段金廒[2] 闫福林[1] 吕洁丽[1] 

机构地区：[1]新乡医学院药学院,河南新乡453003 [2]南京中医药大学江苏省方剂研究重点实验室,南京210046

出　　处：《中国实验方剂学杂志》2012年第12期205-208,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省教育厅自然科学研究计划项目(2011B310006);新乡医学院第七批重点实验室开放课题(ZD2009067);新乡医学院高学历人才科研项目(100779)

摘　　要：目的:通过观察艾叶不同提取物(水提液、95%醇提液、先水提后95%醇提混合液)对痛经模型小鼠的影响,初步探讨艾叶治疗原发性痛经的作用机制。方法:ICR雌性小鼠随机分为9组,分别是正常组、模型组、阳性药西乐葆45.0 mg.kg-1组、艾叶不同提取物的高、低剂量组(1.65,0.55 g.kg-1)。除正常组外,其余各组以10 mg.kg-1的剂量给小鼠sc苯甲酸雌二醇连续7 d,第8天给予ip缩宫素10 mL.kg-1制造原发性痛经模型。各组均在造模第5天起,每天ig给予不同药物连续4d,观察艾叶不同提取物对原发性痛经模型小鼠的扭体次数以及对子宫组织匀浆中一氧化氮(NO)和钙离子(Ca2+)水平的影响。结果:与模型组相比,艾叶醇提物高剂量、先水提后醇提混合液的高、低剂量对原发性痛经小鼠的痛阈有极显著的提高,扭体反应抑制率分别为36.9%,52.5%,42.2%(P<0.01),且相同剂量下先水提后醇提混合液的作用优于水提液(P<0.05);此外艾叶醇提物高剂量、先水提后醇提混合液的高、低剂量组能显著增加子宫组织内NO含量,降低Ca2+含量(P<0.01)。结论:艾叶提取物制法不同,对原发性痛经模型小鼠的镇痛作用强度不同。在3种不同提取物中,先水提后醇提混合液药效最强,醇提液次之,水提液最弱,这可能与其溶出的效应物质不同有关。Objective ： To investigate the influence of the extracts of Folium Artemisiae argyi （FAA） with different extraction methods （by water, by ethanol, firstly by water then by alcohol） on the primary dysmenorrheal model in mice and to clarify the potential mechanism. Method： The ICR mice were randomly divided into nine groups, including normal control group, model group, masculine control group and the extract of FAA extracted with different preparation method groups （ low and high dosage, 0.55, 1.65 g·kg^-1 accordingly）. Except the control group, all the other groups were injected subcutaneously estradiol benzoate （10 mg·kg^-1） with seven consecutive days, and then these mice were injected intraperitoneally oxytocin （10 mL ·kg^-1） at the eighth day so that the primary dysmenorrheal model was established. All the groups were orally administered once pre day with four consecutive days after the model has been made for 5 days. The mouse model of primary dysmenorrheal was used to study the effect of FAA extracted with different preparation methods （low dose and high dose） on writhing response and the levels of nitric oxide （NO） and calcium ion （Ca2＋ ） in mice uterine tissue. Result： The ethanol extract in high dosage of FAA and the water alcohol mixture extract in both dosages of FAA could increase pain threshold markedly, and the inhibitory rate was 36.9% , 52.5% and 42.2% （P 〈 0.01 ） , respectively. Furthermore, the solution of FAA extracted firstly by water then by alcohol showed the stronger effect than the water extract at the same dosage by reducing the writhing times （P 〈 0.05）. In addition, the ethanol extract in high dosage of FAA and the water alcohol mixture extract in both dosages of FAA showed significant effect on primary dysmenorrhea by increasing NO concentration and reducing Ca2＋. concentration in uterine tissue （P 〈 0. 01 ）. Conclusion： The different extracts of FAA showed different antidysmenorrheic trend on primary dysmenorrhea model mi

关 键 词：艾叶 不同提取物 原发性痛经 一氧化氮 钙离子 

分 类 号：R285.5[医药卫生—中药学]